Saikat Chakraborty, Phd Email & Phone Number

Greenfield, In, Us

Indianapolis, Indiana, Us

Indianapolis, Indiana, Us

1. Developed and qualified ddPCR, qPCR, ELISA, and flow-based assays in lentiviral vectors.2. Led a group of scientists and associates, troubleshooting LVV analytical assay development.3. Evaluated new analytical testing methods/tools for suitability in viral vector potency and residual testing.

Basel, Baselstadt, Ch

1. Developed and validated assays for genomic titer determination using ddPCR for ZOLGENSMA® and other Avexis gene therapy products.2. Ensured good documentation practices (GDP) and good laboratory practices (GLP) were followed.3. Demonstrated strong technical writing experience in SOPs and method validation protocols.Gained experience in a GMP environment.

Chicago, Illinois, Us

1. Designed and led mouse in vivo experiments in cognitive neuroscience, pharmacodynamics, and behavioral neuroscience.2. Led optimization of the viral vector (AAV9 and CRISPR Cas9) mediated epigenetic genome editing approach in vitro and in vivo models and pharmacodynamics study in Alzheimer’s disease.3. Demonstrated leadership in establishing CRISPR-CAS9 based gene knockout system, AAV9 based overexpressing system in mouse brain, stable neuronal cell line generation.4. Designed and executed patch clamp electrophysiology study to measure neuronal functional assays in isolated cells and Alzheimer’s brain tissues.5. Performed mouse brain stereotaxic surgeries, in vivo model development, and drug dosing, brain tissue collection.6. Supervised biochemical/molecular analysis of tissues (WB, qPCR, ELISA).7. Led and contributed to writing NIH grants, IACUC protocols, scientific reports, and presenting at international conferences.

Stony Brook, Ny, Us

1. Demonstrated leadership in preclinical pain surgical models development, drug dosing and lentivirus shRNA delivery in spinal cord/DRG neuron, pharmacodynamics, and behavioral assays and tissue collection.2. Accomplished in vitro neuropharmacology researcher by patch clamp electrophysiology functional assays and small molecule drug screening in isolated DRG neuron.3. Designed and optimized lentiviral RNAi knocked down system in vitro and in vivo in pain pathway study.4. Managed projects in novel preclinical in vitro and in vivo oncology model development with A549 lung cancer cell line.

Boston, Ma, Us

1. Optimized Chloride channel CLC3 functional study in GI smooth muscle cells. 2. Designed and completed Chloride channel expression analysis by Western blot and Immunohistochemistry. 3. Initiated and demonstrated protein expression measurements in the presence of Chloride channel Blocker. 4. Demonstrated In vivo pharmacology research in presence of Chloride channel blocker (Niflumic Acid) pharmacodynamics, animal perfusion, tissue harvest, and tissue processing.

Detroit, Mi, Us

1. An accomplished researcher in Molecular cloning and plasmid design, Baculovirus vector construction, qPCR, ELISA.2. Optimized Small to Large-scale Baculovirus purification (Iodixanol /sucrose gradient), protein expression in sf9 expression systems, large scale recombinant protein production.3. Demonstrated leadership in protein expression optimization in the sf9 system and purification using chromatography systems.4. Exhibited skills valued in protein expression in E. coli/ sf9 cells using shake flasks and bioreactor. Protein purification process optimization using gravity flow and FPLC (AKTA) platforms. 5. Assay development troubleshooting (ELISA, enzymatic activity assays, PKC functional assay, etc.). Developed and designed Polyclonal antibodies, Protein conjugation studies.

Indianapolis, In, Us

1. Successfully led cardiovascular preclinical studies, TRPC ion channel Pharmacodynamics research, and Cell and tissue-based assays. 2. Demonstrated leadership skills in vivo electroporation experiments in preclinical high-fat pig models, TRPC6 gene knocked down, and Tissue harvest.3. Optimized Studies in calcium signaling by patch clamp electrophysiology and HTS imaging.4. Non-viral method of genome editing process optimization, application on sh-RNA/si-RNA in gene knockdown.5. Exhibited skills in problem-solving, effective communication, strategic thinking and independently analyzing and interpreting data (e.g., GraphPad Prism).

Tsu, Mie, Jp

1. Large scale Protein/ Enzyme ( Diaphorase) expression in E. coli using shake flasks and bioreactor. Protein purification using gravity flow and FPLC (AKTA) platforms and affinity. Molecular cloning and transformation techniques.2. Assay development (ELISA, Diaphorase/ NADH dehydrogenase enzymatic activity assays).3. Protein analytical methods and techniques, Biochemical and Biophysical characterization of protein.4. DNA, RNA and Plasmid isolation, Gel Electrophoresis, PCR, cDNA and Plasmid vector construction, Ligation, Transformation.

Ph.D, Molecular Biology And Applied Microbiology

Master'S Degree, Microbiology