Saikat Chakraborty, Phd Email & Phone Number

Greenfield, IN, US

Indianapolis, Indiana, US

Indianapolis, Indiana, US

1. Developed and qualified ddPCR, qPCR, ELISA, and flow-based assays in lentiviral vectors.2. Led a group of scientists and associates, troubleshooting LVV analytical assay development.3. Evaluated new analytical testing methods/tools for suitability in viral vector potency and residual testing.

Basel, Baselstadt, CH

1. Developed and validated assays for genomic titer determination using ddPCR for ZOLGENSMA® and other Avexis gene therapy products.2. Ensured good documentation practices (GDP) and good laboratory practices (GLP) were followed.3. Demonstrated strong technical writing experience in SOPs and method validation protocols.Gained experience in a GMP environment.

Chicago, Illinois, US

1. Designed and led mouse in vivo experiments in cognitive neuroscience, pharmacodynamics, and behavioral neuroscience.2. Led optimization of the viral vector (AAV9 and CRISPR Cas9) mediated epigenetic genome editing approach in vitro and in vivo models and pharmacodynamics study in Alzheimer’s disease.3. Demonstrated leadership in establishing CRISPR-CAS9.

Stony Brook, NY, US

1. Demonstrated leadership in preclinical pain surgical models development, drug dosing and lentivirus shRNA delivery in spinal cord/DRG neuron, pharmacodynamics, and behavioral assays and tissue collection.2. Accomplished in vitro neuropharmacology researcher by patch clamp electrophysiology functional assays and small molecule drug screening in isolated.

Boston, MA, US

1. Optimized Chloride channel CLC3 functional study in GI smooth muscle cells. 2. Designed and completed Chloride channel expression analysis by Western blot and Immunohistochemistry. 3. Initiated and demonstrated protein expression measurements in the presence of Chloride channel Blocker. 4. Demonstrated In vivo pharmacology research in presence of.

Detroit, MI, US

1. An accomplished researcher in Molecular cloning and plasmid design, Baculovirus vector construction, qPCR, ELISA.2. Optimized Small to Large-scale Baculovirus purification (Iodixanol /sucrose gradient), protein expression in sf9 expression systems, large scale recombinant protein production.3. Demonstrated leadership in protein expression optimization.

Indianapolis, IN, US

1. Successfully led cardiovascular preclinical studies, TRPC ion channel Pharmacodynamics research, and Cell and tissue-based assays. 2. Demonstrated leadership skills in vivo electroporation experiments in preclinical high-fat pig models, TRPC6 gene knocked down, and Tissue harvest.3. Optimized Studies in calcium signaling by patch clamp electrophysiology.

Tsu, Mie, JP

1. Large scale Protein/ Enzyme ( Diaphorase) expression in E. coli using shake flasks and bioreactor. Protein purification using gravity flow and FPLC (AKTA) platforms and affinity. Molecular cloning and transformation techniques.2. Assay development (ELISA, Diaphorase/ NADH dehydrogenase enzymatic activity assays).3. Protein analytical methods and.

Ph.D, Molecular Biology And Applied Microbiology

Master'S Degree, Microbiology