Sandra Abbott Email & Phone Number

United States

Tarrytown, New York, Us

Boston, Ma, Us

Boston, Ma, Us

Waltham, Massachusetts, Us

Waltham, Massachusetts, Us

Cambridge, Massachusetts, Us

Scientist I- Molecular Biology Baculovirus• Molecular cloning of AAV Rep gene variants into various donor plasmids.• Engineering of gene cassettes for AAV Rep and Cap genes utilizing various baculovirus promotors for evaluation of protein expression ratios to support potency and production of drug products.• Tn7 Translocation of AAV ORF genes into a Bacmid.• Direct cloning into the baculovirus genome. • Transfection of Rep/Cap or Transgene containing Bacmid into Sf9 cells to produce Baculovirus Infected Insect Cells (BIIC).• Direct supervision of one Ph.D. coop student.• New manager training completed October 2019.

Antibody Development and Molecular Biology

Cambridge, Ma, Us

• Protein engineering and biochemical analysis to support early and mid-stage project advancement.• Construct engineering of proteins using various cloning methods, including Gibson, seamless, restriction digest, and infusion cloning.• Transient transfection of individual and co-expressed protein kinases.• Cell line maintenance for transfection.• Stable transfection with both lipofectamine and lentiviral delivery.• Coomassie and western blot (including Licor) analysis of expressed proteins.• Small scale protein purification of tagged proteins.• P100 processing and kinase assays for protein analysis.• Presented experimental results in group meetings.

Boston, Massachusetts, Us

Humanization and engineering of lead candidate antibodies viaPCR, DNA sequence mutation and analysis, cloning heavy andlight chains into individual vectors, and cloning expressionvectors for stable transfection.-Testing antibody variants for expression post-transfection.-Determining antibody sequences from murine and rabbithybridoma cell pellets utilizing PCR and DNA sequencing.-Engineering of Antigen for in vitro and in vivo testing.-Generated small and large scale amounts of protein to supportvarious programs experimental needs.-Superhumanization of antibody sequences determined from hybridomas.-Development of antibody display and discovery technology.-Proficient at transient transfection and stable cell line generation.-Proficient in primer design for implementing DNA mutations andsequencing of full antibodies.-Biacore characterization of antibodies.

Aptamer DiscoveryProficient in both in vitro and cell based SELEX (Systematic Evolution of Ligands by EXponential Enrichment) methods. Utilized these techniques to generate therapeutic aptamers for both internal and partnered programs. Selected several aptamers that are in late stage pre-clinical development. Responsible for screening, characterization, and generation of clones for binding and functional assays. In addition to selection activities, participated in platform enhancement and development initiatives.Prepared technical summaries, protocols and quantitative assays. Maintained accurate and up to date notebooks. Prepared and gave presentations in small or large group settings.Fostered team spirit and unity through participation in team building events and as a member of the Archemix Fun Committee.

Sequencing Facility Dec. 2007-May 2008Employee with Aerotek contracted to Archemix Corp. to run their sequencing facility.Personally responsible for maintenance of the sequencing equipment, ordering supplies, and performing all sequencing related activities for a facility delivering ~4000 sequences/month.Made significant improvements to the sequencing process that saved both time and money.Worked closely with scientists of all levels to ensure timely delivery of high quality data. Was hired as a full time employee in a different department May of 2008.

Division of EndocrinologyConducted research to determine the correlation between extracellular Magnesium levels and cardiac tissue degradation in diabetic patients.Utilized sterile technique and tissue culture skills to fastidiously maintain cell lines used in study. Performed RNA extraction from kidneys, endothelial, and fibroblast cells and prepared cDNA for real time PCR analysis. Ran RNA gel electrophoresis and real-time PCR from fibroblast and endothelial cells as well as from kidney and lung tissue of knock-out mice.Measured Na+/Mg2+ exchanger activity in red blood cells to determine the kinetics of extracellular Mg2+ flux.

Master Of Public Health - Mph, Risk Sciences And Public Policy

Certificate In Risk Sciences And Public Policy, Risk

Bachelor Of Science, Biology

B.S. Biology, Biology, Chemistry, Embryology