Sanjeev Kumar Email & Phone Number

Gurugram, Haryana, India

Conducted wet lab procedures for NGS Solid Tumor Panel using Ion Torrent S5 sequencing platform.Worked on various custom-based and commercial panels, including 52 gene, 161 gene, 500 gene, BRCA germline and somatic panel, and custom panels like (5, 7, 12, 20) gene panels.Conducted sample preparation, quality control, and library construction for sequencing.Collaborated with a team to troubleshoot and optimize protocols and validation for sample preparation and sequencing.Maintained laboratory equipment and supplies, ensuring adherence to laboratory safety protocols.

Gurugram, Haryana, India

Research and Development (R&D)In my role in Research and Development (R&D), I focus on optimizing molecular tools for enhanced performance in diagnostics and research. This includes:Primer Optimization: Developing and refining primers to improve the specificity and sensitivity of PCR assays.In-house Kit Development: Creating custom kits tailored to specific laboratory needs, optimizing their efficiency and reliability.PCR Optimization: Fine-tuning PCR conditions and protocols to maximize amplification efficiency and accuracy.Setting Cut-off Values: Establishing precise cut-off values for tests to ensure accurate interpretation of results.Validation of New Reagents: Thoroughly testing and validating new reagents to verify their performance and reliability in laboratory procedures.My work in R&D contributes to the advancement of molecular diagnostics and supports the development of innovative solutions for research and clinical applications.

Gurugram, Haryana, India

Sanger Sequencing:With a focus on molecular diagnostics, I employ Sanger sequencing to perform a wide array of tests, including MSI, DPYD, TPMT, and JAK 12-15. Sanger sequencing is pivotal in identifying genetic variations, offering insights into disease mechanisms and treatment strategies.PCR:My experience encompasses conducting PCR tests for numerous genetic markers, such as BCR-ABL, ALL, AML, PML, EGFR, KRAS, NRAS, BRAF, JAK-2, and MGMT. PCR serves as a cornerstone in molecular diagnostics, facilitating the sensitive and specific detection of target DNA sequences.Nucleic Acid Extraction:Proficient in extracting nucleic acids (DNA, RNA, cfDNA) from diverse samples, including blood, FFPE tissue, buccal swabs, and plasma. These extracted nucleic acids serve as vital substrates for downstream molecular analyses, providing crucial genetic insights for diagnostic and research purposes.

Gurugram, Haryana, India

Sanger Sequencing:With a focus on molecular diagnostics, I employ Sanger sequencing to perform a wide array of tests, including MSI, DPYD, TPMT, and JAK 12-15. Sanger sequencing is pivotal in identifying genetic variations, offering insights into disease mechanisms and treatment strategies.PCR:My experience encompasses conducting PCR tests for numerous genetic markers, such as BCR-ABL, ALL, AML, PML, EGFR, KRAS, NRAS, BRAF, JAK-2, and MGMT. PCR serves as a cornerstone in molecular diagnostics, facilitating the sensitive and specific detection of target DNA sequences.Nucleic Acid Extraction:Proficient in extracting nucleic acids (DNA, RNA, cfDNA) from diverse samples, including blood, FFPE tissue, buccal swabs, and plasma. These extracted nucleic acids serve as vital substrates for downstream molecular analyses, providing crucial genetic insights for diagnostic and research purposes.

Master'S Degree, Msc Biotechnology

Bachelor'S Degree, Bmlt

Diploma, Dmlt, A+

Activities and Societies: Student Clubs, charities and volunteer work