Teaching piano in primary schools

Metabolite identification using high resolution LC-MS/MS to elucidate the structure of novel metabolites. Analysis of in-vitro and in-vivo samples; mostly radio-labelled, enabling quantitation. HPLC method development using a wide range of columns and mobile phases to enable separation of closely related metabolites.All work carried out to GLP requirements.

Established a successful metabolite identification team and had responsibility for the planning and delivery of metabolite profiling and identification workRange of analyses such as major routes of metabolism to identify metabolic hot spots, cross-species profiling to select suitable toxicology species and identify potential human-specific metabolites, and analysis of in vivo samples for later-stage compoundsExperienced with high-resolution UPLC-MS/MS using Thermo Q-Exactive Orbitrap and Waters Q-Tof instruments, and Xcalibur, Freestyle, Compound Discoverer and Masslynx software. LC-MS method development

NMR support to medicinal chemistry and process chemistry: expertise in structure elucidation of small molecules and quantitative NMR to identify sample purity, stoichiometric ratios and levels of mixture componentsSkilled spectrometer use with thorough knowledge of TopSpin, Icon-NMR, and Vnmr to carry out more complex structural investigationsNMR support to DMPK, determining structures of key metabolites down to the 1ug levelImplementation of novel experiments from the literatureSet up of non-standard experiments to be run under automation, enabling a wide range of experiments to be available to chemistsEvaluation and implementation of new technology, e.g. cryoprobe for detection of sub-ug quantities and capillary flow probe for analysis of samples in 96-well platesInitiated collaboration with clinical DMPK team to quantify circulating metabolites in human plasma samples using NMR with cryoprobe technologySet up procedures to generate and isolate major metabolites in sufficient quantities for structure determination by NMR and biological testingPreparation and delivery of training courses on NMR interpretation using presentations and workshopsUndertook a secondment to the metabolite ID team, using high resolution mass spectrometry to identify metabolites from in vivo and in vitro samples.

Industrial Trainee working in Physical Sciences, specifically physical organic properties