Satish Adhikari, Phd Email & Phone Number

Philadelphia, Pa, Us

Philadelphia, Pennsylvania, Us

Philadelphia, Pennsylvania, Us

University, Ms, Us

• Routinely used Gibson Assembly to create constructs for various purposes such as recombinant protein production, mutant generation and reporter assays.• Extensively used affinity chromatography and size exclusion chromatography to purify recombinant proteins for antibody production, gel shift assay.• Used Next Generation Sequencing methods such as RNA-seq and ChIP-seq to study global gene expression profile. Used RT-qPCR to verify the global gene expression profile. • Performed immunoprecipitation (IP) and mass spectrometry (MS) to study protein-protein interaction. Used bacterial two hybrid assay to verify the interaction.• Used confocal microscopy to study gfp and mcherry tagged protein localization.• Mentored undergraduate students in their final year thesis.

University, Ms, Us

• Taught several laboratory courses to undergraduate students.• Gained invaluable experience in mentoring students with various needs and learning abilities.• Guest lecturer in advanced microbiology class for undergraduate students.

Balkumari, Lalitpur, Bagmati, Np

• Involved in setting up in a new research lab. Responsibilities included optimizing protocols for several new equipments. • Trained new employees on using the lab equipment such as thermal cycler, gel electrophoresis unit, SDS PAGE.• Involved in isolation and characterization of novel yeast strains.• Involved in several projects with varying responsibilities including designing and performing experiments. Other responsibilities include writing, and presenting lab reports and supervising junior researchers.

Doctor Of Philosophy - Phd, Microbiology, General

Master Of Science (Ms), Biotechnology

Bachelor Of Technology (B.Tech.), Biotechnology