Satish Adhikari, Phd work email
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Satish Adhikari, Phd personal email
- More than 10 years of research experience in molecular biology techniques in academia and industry.- Extensive knowledge and experience in plasmid design for molecular cloning, protein/antibody expression, and viral vectors. - Highly experienced in design, production, and purification of virus-like particles (VLPs) for protein production and targeted delivery.
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Manager - Platform DevelopmentChimeron Bio Jan 2024 - PresentPhiladelphia, Pennsylvania, Us -
ScientistChimeron Bio Apr 2022 - Dec 2023Philadelphia, Pennsylvania, Us
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Research AssistantUniversity Of Mississippi May 2016 - Dec 2021University, Ms, Us• Routinely used Gibson Assembly to create constructs for various purposes such as recombinant protein production, mutant generation and reporter assays.• Extensively used affinity chromatography and size exclusion chromatography to purify recombinant proteins for antibody production, gel shift assay.• Used Next Generation Sequencing methods such as RNA-seq and ChIP-seq to study global gene expression profile. Used RT-qPCR to verify the global gene expression profile. • Performed immunoprecipitation (IP) and mass spectrometry (MS) to study protein-protein interaction. Used bacterial two hybrid assay to verify the interaction.• Used confocal microscopy to study gfp and mcherry tagged protein localization.• Mentored undergraduate students in their final year thesis. -
Graduate Teaching AssistantUniversity Of Mississippi Aug 2014 - May 2016University, Ms, Us• Taught several laboratory courses to undergraduate students.• Gained invaluable experience in mentoring students with various needs and learning abilities.• Guest lecturer in advanced microbiology class for undergraduate students.
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Research AssociateResearch Institute For Bioscience & Biotechnology (R.I.B.B) Dec 2011 - Jul 2014Balkumari, Lalitpur, Bagmati, Np• Involved in setting up in a new research lab. Responsibilities included optimizing protocols for several new equipments. • Trained new employees on using the lab equipment such as thermal cycler, gel electrophoresis unit, SDS PAGE.• Involved in isolation and characterization of novel yeast strains.• Involved in several projects with varying responsibilities including designing and performing experiments. Other responsibilities include writing, and presenting lab reports and supervising junior researchers.
Satish Adhikari, Phd Skills
Satish Adhikari, Phd Education Details
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University Of MississippiGeneral -
Uppsala UniversityBiotechnology
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Kathmandu UniversityBiotechnology
Frequently Asked Questions about Satish Adhikari, Phd
What company does Satish Adhikari, Phd work for?
Satish Adhikari, Phd works for Chimeron Bio
What is Satish Adhikari, Phd's role at the current company?
Satish Adhikari, Phd's current role is Manager - Platform Development.
What is Satish Adhikari, Phd's email address?
Satish Adhikari, Phd's email address is sa****@****iss.edu
What schools did Satish Adhikari, Phd attend?
Satish Adhikari, Phd attended University Of Mississippi, Uppsala University, Kathmandu University.
What skills is Satish Adhikari, Phd known for?
Satish Adhikari, Phd has skills like Sds Page, Pcr, Dna Extraction, Molecular Biology, Gel Electrophoresis, Elisa, Biochemistry, Western Blotting.
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