Abu Sayeed Mohammad Mahmud Email & Phone Number

Lucas Heights, New South Wales, Australia

Sydney, New South Wales, Australia

1) Investigate OOS / OOT, deviations, and laboratory testing events with root cause analysis and implement CAPA.2) Introduction of Steritest Symbio Pumps for Sterility Testing of Radiopharmaceutical Products.3) Endotoxin testing using KQCL methods for radiopharmaceutical products and the introduction of Next-generation PTS methods for endotoxin testing.

Parkville, Victoria, Australia

• Develops figures, does literature searches, assists with manuscript writing submit abstracts, and presents findings at regional and national meetings.• Develop, adapt, and implement new research techniques and protocols• Assist in preparation of grants application, designs, and performs experiments

Dhaka, Bangladesh

• Provide critical computational medical research leadership and performed an essential role in assessing and implementing solutions to mitigate research challenges.• Perform general office management, manage research projects, timeframe, deliverables, curating and communicating research data for industry, government, and public, including interpreting research findings and summarizing data into reports.• Development and analysis of biochemical assays to aid target characterization using ELISA, FACS, flow cytometry (DS CytoFLEX LX), and Western blotting to detect, quantify, and study the binding, expression, or activity of biomolecules, such as enzymes, proteins (protein-protein, protein-RNA, and protein-DNA), and small molecules.• Facilitate several successful projects, including whole-genome sequencing (WGS) of SARS-CoV-2 Bangladeshi isolates. Engaged in wet-lab & bioinformatic analysis of over 700 SARS-CoV-2 whole-genome and published 616 SARS-CoV-2 WGS in GISAID and NCBI.• Detect viruses by a metatranscriptomic approach using 16S rRNA gene, virus neutralization (observing cytopathic effect), hemagglutination inhibition, ELISA, PCR, LAMP, and RT-qPCR based assays.• Mouse model; Isolated, identified and characterized Lactococcus lactis strain, observed their probiotic potential against V. cholerae induced diarrhea in Mouse model.• Developed ELISA-based toxin assays for the detection of aflatoxins, ochratoxin A, patulin, deoxynivalenol from different foods collected from the local market,• Created, maintained, and passaged mammalian cell lines, conducted transfection by electroporation and chemical methods.• Experienced in next-generation sequencing technologies and data analysis; sequenced over 200 Bacterial whole genomes, including E. coli, Salmonella typhi, Enterobacter species, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii.

Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka, Bangladesh

• Examined blood, tissue, urine, stools, CSF, and other specimens to investigate pathogens (bacteria, viruses, fungi, and parasites) that cause diseases using a variety of microbiological, biochemical, immunological, and molecular methods.• Developed protocols for the detection of viruses including coronavirus (SARS-CoV-2), Avian influenza (H5N1), White spot syndrome & Myonecrosis virus, and GMO products, using LAMP, PCR, qPCR, and RT-PCR. Provided training on PCR and RT-PCR from 2012 to 2014.• Performed molecular biology-based techniques including, but not limited to, gel electrophoresis, pulse-field gel electrophoresis (PFGE), field inversion gel electrophoresis (FIGE), PCR amplification, qPCR, RT-PCR, from clinical specimens containing potential human pathogens.• Performed library construction in preparation for DNA sequencing. Techniques included Nextera library preparation technology, quantification, and qPCR. Interacted with, loaded, and maintained an Illumina MiniSeq, NextSeq 550, and NovaSeq 6000 system sequencer and BaseSpace Sequencing Hub platform.• Executed bioinformatic analysis using the pipeline for reads quality control (Trimmomatic), reference strain determination (Mash), read mapping to reference strain (BWA), SNPs and Indel detection (Samtools, Varscan2), de novo genome assembly (SPAdes), genome annotation (Prokka), phylogenetic tree construction (MEGAX, RAxML), & phylogenetic analysis (PAML).• Supervised laboratory staff for daily laboratory operations, including arranging lab duties. Also, supervised 4 Honors project students and 6 MS thesis students within the allotted budget and timelines.

St. Albans, Victoria, Australia

Utilize interpersonal skills to facilitate lectures to students on medical microbiology and immunity at a leading university campus specializing in health and nutrition research. Contribute to curriculum development and design of subjects and resources. Contribute to curriculum development and design of subjects and resources. Developed and implemented successful academic projects from conception to completion.  Observation of living microorganisms, the separation of microbes, asepsis, human and environmental microflora, and differential staining gram stain, the acid-fast stain, endospore, and capsule. Challenge and motivate students using blended/hybrid course design structure. Conduct needs assessments and direct students to educational resources to help facilitate their learning process. Commended for maintaining enthusiasm, tenacity, intense focus, and dependability during the educational process.

Dhaka, Bangladesh

• Calibrated equipment and qualification support (IQ, OQ, PQ) based on internal and external standards.• Participated in validation activities (analytical methods, cleaning, & process), qualification & validation (equipment, system, facilities) based on internal/external standards, established validation schedules, built databases to track validation activities.• Validated FTM, CTM, SCDB, TSB broth (growth promotion test), suitability testing in the presence of the product.• Validated process of new product and raw material changes, determined bioburden for validation of sterilisation, production, and cleaning processes.• Validated analytical methods, including sterility/LAL test, antimicrobial potency, and preservative efficacy testing.• Validated computer systems requirements and overhauled QC lab electronic & hardcopy data & records.• Validated 90% of computer systems requirements as per GAMP5 and 21 CFR Part 11 compliant and overhauled QC lab electronic & hardcopy data & records.• Generated, and preserved 100% of matrices revised, analysed trends and results regarding product inspection in all stages of the process in search of improvements based on metrics and thus ensuring the quality of products.• Performed Risk Assessments, Corrective and Preventative (CAPA) investigations, raise Deviation and Incident Reports (DIR), Investigated out of specifications (OOS) results, Out of Limits forms (OOL) and initiate Change Control (CC) when necessary engaged in root cause analysis (RCA) using quality risk management (QRM) tools, including basic risk management facilitation methods (flowcharts, check sheets, fishbone diagram), failure mode effects analysis, and ensuring timely completion of CAPA.• Reviewed 90% manufacturing batch records and QC batch testing reports for compliance to cGMP and product license and participated in the development of electronic batch record (EBR) review system.

Tejgaon I/A, Dhaka, Bangladesh

• Performed microbial testing, recording, and reporting for starting materials, bulk products, finished goods, production water as per SOPs and protocols developed in compliance with TGO100 (Microbiological Standards for Medicines), BP and Harmonised Pharmacopoeia (USP/EP/JP).• Prepared media for routine tests, performed growth promotion test of new batches of media purchased, suitability testing in the presence of the product, and preserving bacterial mother cultures and subcultures.• Monitored and maintained environmental microorganisms (contact plate, swab, Finger dab) and particles (sampling from a different location and testing) to ensure Cleanliness Classes in Cleanrooms and Clean Zones as per ISO 14644/US FS209E. • Specified pathogens detection based on Bergey’s manual of determinative bacteriology (morphology, culture, biochemical test), and Bergey’s manual of systematic bacteriology (16s rRNA sequencing/ metabarcoding).• Bacteria identification by 16S rRNA sequencing, microscopy, growth characteristics on plates, Biolog plates and API kits, Vitek 2 compact and Vitek Mass Spectrometry identification systems.• Carried out pH, Salinity, Dissolved Oxygen, Turbidity, TSS, TDS, Temperature, Flow Rate, Chloride, Nitrates, Phosphorous, Ammonia, Faecal Coliform, BOD, TOC, pesticides, and heavy metals testing using water analysis kits, Total organic carbon analyser, BOD robot, Ion chromatography (COD), Flow injection analysis instrument, UV Spectrometer, HPLC, and AAS.• Enumerated microorganisms (limit test), including total coliforms, using membrane filtration, conventional plate count (TVC, TBC, TAMC, TYMC), and MPN methods in water, oral drugs, raw materials, and finished goods.• Performed sterility testing (direct/indirect methods), and endotoxin limit (LAL) test.

Gazipur, Dhaka, Bangladesh

• Enumerated microorganisms (limit test), including total coliforms, using membrane filtration, conventional plate count (TVC, TBC, TAMC, TYMC), and MPN methods in water, oral drugs, raw materials, and finished goods.• Performed sterility testing (direct/indirect methods), and endotoxin limit (LAL) test.• Performed antibiotic sensitivity test (Disk diffusion/ Epsilometer testing), antibiotic potency test using diffusion (Cylinder-Plate) method, and preservative efficacy test (ISO 11930/ USP <51>).• Mentored new colleagues to improve their engagement, promote a positive workplace atmosphere and skilled employee. Conducted training in aseptic techniques, aseptic gowning, environmental monitoring, and cleanroom behaviour to personnel accessing the aseptic area. • Executed the media fills program including the writing of protocol, script, performing quality oversight, containers, vials incubation, inspection and final report writing. Conducted Media simulation test to ensure aseptic filling.• Active participant in 90% internal quality auditing to comply cGMP & FDA pre-approval inspection, prepare compliance reports and follow up audit observations, organised investigation and closure of deviations with comprehensive root cause analysis and ensuring timely completion of CAPA.

Doctor Of Philosophy - Phd, Medical Bacteriology And Proteomics, Passed

Activities and Societies: Awards: • Endeavor Postgraduate Scholarship 2015 – 2019 • Top-Up Scholarship (Oral Health CRC) 2015 – 2018 •.

Masters Of Science (Ms), Microbiology, 3.67

Thesis Title: Reduced susceptibility to fluoroquinolone in Salmonella serovar Typhi and molecular aspect of fluoroquinolone resistance.

Bachelor Of Science (B.Sc.), Microbiology, 3.76

Activities and Societies: Chittagong University Scholarship (University of Chittagong) 2006Project Topic: Implementation.