Abu Sayeed Mohammad Mahmud Email & Phone Number
Who is Abu Sayeed Mohammad Mahmud? Overview
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Abu Sayeed Mohammad Mahmud is listed as Microbiologist at ANSTO, a company with 977 employees, based in Sydney, New South Wales, Australia. AeroLeads shows a matched LinkedIn profile for Abu Sayeed Mohammad Mahmud.
Abu Sayeed Mohammad Mahmud previously worked as Sterility Assurance Microbiology Specialist at Ansto and Honorary (Fellow) at University Of Melbourne. Abu Sayeed Mohammad Mahmud holds Doctor Of Philosophy - Phd, Medical Bacteriology And Proteomics, Passed from University Of Melbourne.
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About Abu Sayeed Mohammad Mahmud
Molecular microbiologist with experience in molecular methods, metagenomic, recombinant technologies, proteomics, NGS, bioinformatics, and cutting-edge research efforts to learn about the microbiome and disease. Well-developed analytical skills and a keen eye for identifying and implementing research strategies for institutions.• Secured 4 competitive research grants, including a $33,195 Special Allocation Grant, $3,416 R&D grant, $3000 Grant Application Working Group-Postgraduate (GAWG PG) the University of Melbourne, and $ 8000 Australian Dental Research Foundation, Inc.• Completed Whole-genome sequencing of 29 Pseudomonas aeruginosa, and 11 Acinetobacter baumannii from patient blood and stool samples, 100 multi-drug resistant E. coli and Salmonella typhi isolated from hospital wastewater.• Facilitated several successful projects, including implementing whole-genome sequencing of SARS-CoV-2 (sequenced over 800 SARS-CoV-2 and published sequencing data in GISAID), resistome analysis, and identifying co-infecting bacterial pathogens along with the SARS-CoV-2 infection and their possible role in influencing the severity of the COVID-19.• Responsible for DNA sequence-based data analysis. Used different platforms (BaseSpace, IDSeq, Galaxy AU/US/EU) & tools (CLC Workbench, Geneious prime) to trim, assemble, map, and detect SNPs/DIPs in genomic DNA data to assess population variation between closely related organisms.• Constructed vectors for efficient expression and secretion of His-tagged proteins/His-tagged with thrombin-cleavage site proteins expression and secretion in bacteria.• Designed different types of His affinity tagged recombinant proteases for easier purification.• Designed, produced, and purified recombinant His-affinity tagged propeptides (rRgpA-PP, rRgpB-PP, rKgp-PP) in E. coli.• Carried out production, purification, characterization of protein, and protein sequencing.• Developed a proteolytic activity assay for protease detection and kinetics studies, which included calculating Km, Vmax, Kcat, Kass, Kdis, Kd, Ki, and IC50, as well as distinguishing between different inhibitory types.• Performed molecular biology-based techniques including, but not limited to, gel electrophoresis, PFGE, FIGE, PCR, qPCR, RT-PCR, from clinical specimens containing potential human pathogens.• Engaged in development and analysis of biochemical assays to aid target characterization using ELISA, FACS, flow cytometry, and Western blotting to detect, quantify, and study the binding, expression, or activity of biomolecules.
Listed skills include Leadership, Cell Biology, Elisa, Molecular Typing, and 49 others.
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Abu Sayeed Mohammad Mahmud work experience
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Microbiologist
1) Investigate OOS / OOT, deviations, and laboratory testing events with root cause analysis and implement CAPA.2) Introduction of Steritest Symbio Pumps for Sterility Testing of Radiopharmaceutical Products.3) Endotoxin testing using KQCL methods for radiopharmaceutical products and the introduction of Next-generation PTS methods for endotoxin testing.
Honorary (Fellow)
- Develops figures, does literature searches, assists with manuscript writing submit abstracts, and presents findings at regional and national meetings.
- Develop, adapt, and implement new research techniques and protocols
- Assist in preparation of grants application, designs, and performs experiments
Senior Scientific Officer
- Provide critical computational medical research leadership and performed an essential role in assessing and implementing solutions to mitigate research challenges.
- Perform general office management, manage research projects, timeframe, deliverables, curating and communicating research data for industry, government, and public, including interpreting research findings and.
- Development and analysis of biochemical assays to aid target characterization using ELISA, FACS, flow cytometry (DS CytoFLEX LX), and Western blotting to detect, quantify, and study the binding, expression, or activity.
- Facilitate several successful projects, including whole-genome sequencing (WGS) of SARS-CoV-2 Bangladeshi isolates. Engaged in wet-lab & bioinformatic analysis of over 700 SARS-CoV-2 whole-genome and published 616.
- Detect viruses by a metatranscriptomic approach using 16S rRNA gene, virus neutralization (observing cytopathic effect), hemagglutination inhibition, ELISA, PCR, LAMP, and RT-qPCR based assays.
- Mouse model; Isolated, identified and characterized Lactococcus lactis strain, observed their probiotic potential against V. cholerae induced diarrhea in Mouse model.
Scientific Officer
- Examined blood, tissue, urine, stools, CSF, and other specimens to investigate pathogens (bacteria, viruses, fungi, and parasites) that cause diseases using a variety of microbiological, biochemical, immunological, and.
- Developed protocols for the detection of viruses including coronavirus (SARS-CoV-2), Avian influenza (H5N1), White spot syndrome & Myonecrosis virus, and GMO products, using LAMP, PCR, qPCR, and RT-PCR. Provided.
- Performed molecular biology-based techniques including, but not limited to, gel electrophoresis, pulse-field gel electrophoresis (PFGE), field inversion gel electrophoresis (FIGE), PCR amplification, qPCR, RT-PCR, from.
- Performed library construction in preparation for DNA sequencing. Techniques included Nextera library preparation technology, quantification, and qPCR. Interacted with, loaded, and maintained an Illumina MiniSeq.
- Executed bioinformatic analysis using the pipeline for reads quality control (Trimmomatic), reference strain determination (Mash), read mapping to reference strain (BWA), SNPs and Indel detection (Samtools, Varscan2).
- Supervised laboratory staff for daily laboratory operations, including arranging lab duties. Also, supervised 4 Honors project students and 6 MS thesis students within the allotted budget and timelines.
Academic Sessional
Utilize interpersonal skills to facilitate lectures to students on medical microbiology and immunity at a leading university campus specializing in health and nutrition research. Contribute to curriculum development and design of subjects and resources. Contribute to curriculum development and design of subjects and resources. Developed and implemented.
Senior Microbiologist
- Calibrated equipment and qualification support (IQ, OQ, PQ) based on internal and external standards.
- Participated in validation activities (analytical methods, cleaning, & process), qualification & validation (equipment, system, facilities) based on internal/external standards, established validation schedules, built.
- Validated FTM, CTM, SCDB, TSB broth (growth promotion test), suitability testing in the presence of the product.
- Validated process of new product and raw material changes, determined bioburden for validation of sterilisation, production, and cleaning processes.
- Validated analytical methods, including sterility/LAL test, antimicrobial potency, and preservative efficacy testing.
- Validated computer systems requirements and overhauled QC lab electronic & hardcopy data & records.
Microbiologist
- Performed microbial testing, recording, and reporting for starting materials, bulk products, finished goods, production water as per SOPs and protocols developed in compliance with TGO100 (Microbiological Standards for.
- Prepared media for routine tests, performed growth promotion test of new batches of media purchased, suitability testing in the presence of the product, and preserving bacterial mother cultures and subcultures.
- Monitored and maintained environmental microorganisms (contact plate, swab, Finger dab) and particles (sampling from a different location and testing) to ensure Cleanliness Classes in Cleanrooms and Clean Zones as per.
- Specified pathogens detection based on Bergey’s manual of determinative bacteriology (morphology, culture, biochemical test), and Bergey’s manual of systematic bacteriology (16s rRNA sequencing/ metabarcoding).
- Bacteria identification by 16S rRNA sequencing, microscopy, growth characteristics on plates, Biolog plates and API kits, Vitek 2 compact and Vitek Mass Spectrometry identification systems.
- Carried out pH, Salinity, Dissolved Oxygen, Turbidity, TSS, TDS, Temperature, Flow Rate, Chloride, Nitrates, Phosphorous, Ammonia, Faecal Coliform, BOD, TOC, pesticides, and heavy metals testing using water analysis.
Executive Microbiologist
- Enumerated microorganisms (limit test), including total coliforms, using membrane filtration, conventional plate count (TVC, TBC, TAMC, TYMC), and MPN methods in water, oral drugs, raw materials, and finished goods.
- Performed sterility testing (direct/indirect methods), and endotoxin limit (LAL) test.
- Performed antibiotic sensitivity test (Disk diffusion/ Epsilometer testing), antibiotic potency test using diffusion (Cylinder-Plate) method, and preservative efficacy test (ISO 11930/ USP <51>).
- Mentored new colleagues to improve their engagement, promote a positive workplace atmosphere and skilled employee. Conducted training in aseptic techniques, aseptic gowning, environmental monitoring, and cleanroom.
- Executed the media fills program including the writing of protocol, script, performing quality oversight, containers, vials incubation, inspection and final report writing. Conducted Media simulation test to ensure.
- Active participant in 90% internal quality auditing to comply cGMP & FDA pre-approval inspection, prepare compliance reports and follow up audit observations, organised investigation and closure of deviations with.
Colleagues at ANSTO
Other employees you can reach at ansto.gov.au. View company contacts for 977 employees →
Livia Salvati Manni
Colleague at Ansto
Australia, Australia
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JB
J Bourke
Colleague at Ansto
Wollongong Area, Australia
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RH
Rhiarn Hoban
Colleague at Ansto
Millers Point, New South Wales, Australia, Australia
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AF
Abdul Fneiche
Colleague at Ansto
Narwee, New South Wales, Australia, Australia
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NM
Nathan Markovski
Colleague at Ansto
Greater Sydney Area, Australia
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MP
Mark Peterson
Colleague at Ansto
Australia, Australia
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CC
Con Cotsovolos
Colleague at Ansto
Greater Sydney Area, Australia
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GT
Gabby Timlin
Colleague at Ansto
Wollongong, New South Wales, Australia, Australia
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MG
Mark Gill
Colleague at Ansto
Greater Sydney Area, Australia
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SG
Sam Groeller
Colleague at Ansto
Wollongong, New South Wales, Australia, Australia
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Abu Sayeed Mohammad Mahmud education
Doctor Of Philosophy - Phd, Medical Bacteriology And Proteomics, Passed
Masters Of Science (Ms), Microbiology, 3.67
Bachelor Of Science (B.Sc.), Microbiology, 3.76
Frequently asked questions about Abu Sayeed Mohammad Mahmud
Quick answers generated from the profile data available on this page.
What company does Abu Sayeed Mohammad Mahmud work for?
Abu Sayeed Mohammad Mahmud works for ANSTO.
What is Abu Sayeed Mohammad Mahmud's role at ANSTO?
Abu Sayeed Mohammad Mahmud is listed as Microbiologist at ANSTO.
Where is Abu Sayeed Mohammad Mahmud based?
Abu Sayeed Mohammad Mahmud is based in Sydney, New South Wales, Australia while working with ANSTO.
What companies has Abu Sayeed Mohammad Mahmud worked for?
Abu Sayeed Mohammad Mahmud has worked for Ansto, University Of Melbourne, Bangladesh Council Of Scientific And Industrial Research (Bcsir), Victoria University, and Monomedi Bangladesh Ltd.
Who are Abu Sayeed Mohammad Mahmud's colleagues at ANSTO?
Abu Sayeed Mohammad Mahmud's colleagues at ANSTO include Livia Salvati Manni, J Bourke, Rhiarn Hoban, Abdul Fneiche, and Nathan Markovski.
How can I contact Abu Sayeed Mohammad Mahmud?
You can use AeroLeads to view verified contact signals for Abu Sayeed Mohammad Mahmud at ANSTO, including work email, phone, and LinkedIn data when available.
What schools did Abu Sayeed Mohammad Mahmud attend?
Abu Sayeed Mohammad Mahmud holds Doctor Of Philosophy - Phd, Medical Bacteriology And Proteomics, Passed from University Of Melbourne.
What skills is Abu Sayeed Mohammad Mahmud known for?
Abu Sayeed Mohammad Mahmud is listed with skills including Leadership, Cell Biology, Elisa, Molecular Typing, Western Blotting, In Vivo, Animal Models, and Proteomics.
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