Sharmeen Chagani, Ph.D. Email & Phone Number

Cell culture: Experienced working with primary and cancer cell lines performing various in vitro experiments to determine cell viability, proliferation, migration, high through put screens, transfection, FACS, luciferase assays & immunocytochemical staining.In vivo modeling: Specialized training in handling mice for administering I.V, I.P, SC injections and oral gavage, development of several xenograft and transgenic models for testing efficacy of developed formulations and performing dissection studies and adept at collecting lymph nodes, skin samples, and isolating organs for analytical measurements, subjecting UV, breeding and genotyping. Adept in imaging techniques like IVIS Lumina system for live animal imaging.Molecular biology: standard molecular biology techniques (RNA/DNA extraction, qPCR, etc.), CHIP-seq, stable/transient gene transfections, Western blots, CRISPR.Histology: Tissue fixation, processing, embedding, microtomy and stainings like Fontana Masson (melanin) staining, immunohistochemical analysis through IHC, CEM staining, immunocytochemistry, immunofluorescence, laser capture microdissection (LCM) of frozen and paraffin embedded tissueStatistical analysis and softwares: Graphpad Prism, Excel, GIMP and Adobe Photoshop/ Illustrator for data processing and analysis and proficient in M.S Office and making power point presentations.

Listed skills include Cell Culture, Molecular Biology, Western Blotting, Cell Biology, and 10 others.