Scott Campbell Email & Phone Number

San Diego

Designed and constructed a plasmid template for in-vitro transcription of mRNA vaccines. Included literature research, using sequence editing software such as DNA Star and Snap Gene, primer design, writing protocols, and executing multiple experiments using Gibson assembly, colony PCR, Maxi Prep, DNA extraction/purification, gel electrophoresis, restriction digest, and sequencing. Developed methods to generate and purify mRNA vaccines from a plasmid template, which involved literature research, writing protocols, in-vitro transcription, DNA purification, and RNA purification via oligo dT chromatography on an AKTA Avant liquid chromatography system. Designed and optimized a process to generate a CHIKV-Luciferase reporter virus from a plasmid template. Involved restriction digests, agarose and formaldehyde gel electrophoresis, in-vitro transcription, spin-column purification, phenol:chloroform extraction, and RNA electroporation. Also included BSL-2 level virus work including virus expansions, plaque assays, and CHIKV neutralization assays to characterize newly generated lots of CHIKV-Luciferase virus. Generated and qualified a GxP lot of virus to be used in phase III virus neutralization assays. Developed a process to sequence the RNA genome of a recombinant CHIKV-Luciferase reporter virus, which involved viral RNA extraction, RT-PCR, PCR, gel extraction, primer design, sequence assembly, and analysis. Characterized recombinant Adenovirus vaccine candidates for HIV by PCR, qPCR, Western blot, sequencing, restriction digest/gel electrophoresis, and viral growth curves. Developed and performed cell-based neutralization assays to determine the NT80 titer of neutralizing antibodies in human serum samples for phase II and phase III clinical trials of a Chikungunya (CHIKV) VLP vaccine candidate. Developed human and NHP ELISAs to determine the titer of CHIKV reactive IgG and IgM antibodies.

Waltham, Ma

Worked in a BSL-2 FDA/CLIA registered clinical testing lab performing infectious disease testing on human organ and tissue transplant donors. All testing followed cGLP and cGDP.I started before the lab was set up, so I was involved in performing operational and procedural qualifications, method validations, equipment correlation studies, installing and calibrating instruments, and planning the laboratory layout.Once the lab opened, we had daily testing runs of human blood, serum, and plasma samples for Hepatitis B, Hepatitis C, HIV, HTLV, Epstein Bar Virus, Cytomegalovirus, West Nile Virus, Toxoplasma, Syphilis, and ABO blood types. ELISA assays:HBs Ag, HBc Total Ab, HBc IgM, HCV Ab, HIV 1-2 Plus O Ab, HTLV I/II, EB VCA IgG, EB VCA IgM, CMV IgG, CMV IgM, Toxoplasma IgG, and nPOD.Nucleic Acid Testing (NAT):HBV, HCV, HIV, and WNV on a high throughput Procleix Tigris instrument.Antibody Agglutination:ABO/Rh and A1 subtyping, CMV Total Ab, and the ASI RPR Card Test for Syphilis. Other Responsibilities:Aside from testing, my other responsibilities included entering test results into the LIMS; generating result reports for clients; daily review of testing paperwork and timesheets; monthly CAP and internal proficiency testing; test kit and reagent lot acceptance (validation); writing and revising SOPs; logging test control data; training and supervising new technicians; communicating and resolving issues with clients; sample receiving, accessioning, and archiving; equipment maintenance; inventory; mixing test reagents and wash solutions; pipetting, diluting, and centrifuging samples; and shipping samples to send out labs.

Honolulu, Hi

Worked with a team of lab assistants to process and package units of human blood, platelets, and plasma. Communicated with hospital staff to fill and ship orders for blood, plasma, and platelets, using knowledge of the ABO blood types and antigen compatibility to suggest alternatives when specific blood types were unavailable. Identified units unsuitable for transfusion due to fibrin, blood clots, bilirubin accumulation, or agglutination. Trained three other lab assistants. Kept accurate and detailed records and performed all tasks according to cGMPs and the relevant SOP. Ensured all units were properly stored by monitoring freezer, refrigerator, and incubator temperatures. Used aseptic technique to draw RBC and plasma samples for rare antigen testing. Calibrated scales, thermometers, and hematology analyzers. Prepared daily inventory reports.

Honolulu, Hi

Performed literature research and lab work on multiple projects for the design, construction, and testing of plasmid DNA vectors for mammalian gene transfer and gene therapy. Worked independently to construct a piggybac transposon plasmid vector resulting in a two-fold increase in transposition activity over previously used vectors. Used prokaryotic cell transformation and cloning, electroporation, plasmid isolation (mini-prep and maxi-prep) and BAC isolation, spectrophotometry, gel DNA extraction and DNA purification, and PCR to amplify the transposon gene, promoter region, mammalian selection cassette, GFP cassette, introns, and TREs from separate plasmids. Used overlap-extension PCR to introduce a hyperactive mutation in the piggybac promoter region. Used restriction digests and ligation, TA cloning, and Gateway recombineering to assemble the fragments into a single 19 kbp plasmid. Tested transposition efficiency in mammalian cells by transfecting HEK 293 cells with the plasmid, extracting RNA, making cDNA, and running qPCR to determine transposon gene expression. Then used SDS-PAGE, and Western blotting to verify transposase size. Used restriction digests and gel electrophoresis for fragment analysis, and DNA sequencing for sequence verification at each stage of the construction. Prepared various selective and differential media, antibiotics, and reagents.

Honolulu, Hi

Worked at an analytical chemistry laboratory during summer break in college. We tested various food, beverage, and waste-water samples for a variety of substances. Used liquid-liquid extraction to analyze samples for percent oil and grease, total petroleum hydrocarbon, and total fat content. Used spectrophotometry to analyze samples for total suspended solids and total dissolved solids. Used titration and stoichiometric calculations to analyze samples for silica, phosphate, fluoride, nitrate, and nitrite concentration, total chloride, citric acid, total phosphorous, and CO2. Used PCR and growth on selective media for detection of E. coli, Listeria sp., and Salmonella sp. Used conductivity meters to measure carbon oxygen demand, dissolved oxygen, and biological oxygen demand. Performed the Kjeldahl method to analyze samples for total Kjeldahl nitrogen.

Kailua-Kona, Hi

Worked at an aquaculture research and development company to develop strains of domesticated tiger prawns. Supervised four hatchery technicians and was responsible for raising large numbers of prawn larvae through the nauplii, zoea, mysis, and post-larval stages, which required careful coordination of tasks with other technicians, and excellent time management. Designed and implemented improvements to hatchery protocols that resulted in a 30% increase in survival rates. Performed microscopic observation of prawn health, adhered to strict schedules for feeding, antibiotic treatment, and water changes. Cultured Chaetoceros sp. and Tetraselmis sp. micro-algae, as well as copepod and artemia micro-feeds. Performed daily testing of water salinity, pH, dissolved oxygen, temperature, NH3, and NO2/NO3 levels. Calculated egg fertility rates, spawn sizes, and hatch rates. Traveled to attend a two-week course in “Engineering Re-Circulating Aquaculture Systems” presented by Cornell University.