Shen Li Email & Phone Number

Research in the Posttranslational modifications of FOXA1 and their roles in ER+ breast cancer cells -- Revealed novel phosphorylation and acetylation sites of FOXA1 in E2 or TNFa-stimulated MCF7 breast cancer cells using immunoprecipitation coupled with LC-MS/MS-- Established 14 CRISPR KI cell lines mimicking the discovered PTMs for the functional studies-- Found the acetylation of FOXA1 affects the growth, migration, and chemoresistance of cancer cells Demonstrated the acetylation of FOXA1 resulted in its genomic relocalizations from FOXA1 ChIP-seq-- Revealed that acetylation of FOXA1 dampened Estrogen signaling pathway and its absence elevated EGFR and TGF-b signaling pathway, driving the gene expression responsible for tamoxifen resistance-- Confirmed the acetylation of FOXA1 affects metastasis and chemoresistance of mouse tumor xenograft-- Implemented CRISPR/Cas-based Prime Editing in breast cancer research with success

Research on Mismatch tolerance during homologous recombination in mammalian cells-- Analyzed the intra-chromosomal recombination involving highly diverged DNA sequences-- Brought to light that the DNA double-strand breaks induced recombination and spontaneous recombinationcarried out exchange between highly diverged sequences, while the former has weak homology requirement-- Confirmed the DNA strands from highly diverged sequences formed heteroduplex DNA intermediates during DNAdouble-strand breaks induced recombination-- Designed a novel recombination substrate to reveal the mechanism of stringent homology requirements inspontaneous recombination-- Examined the roles of BLM and RecQ4 in DNA double-strand break repairTeaching in Biology, Anatomy and Physiology Labs

Beijing Suburb, China

Mouse Genetics-- Designed and generated transgenic mice, knock-in and conditional knockout mice for genetic studies in autophagy, diabetes, and endocrinology-- Studied histological, behavioral, and hormonal changes in genetically modified mice

Doctor Of Philosophy (Phd), Molecular Genetics

Research Assistant in Molecular Genetics: mechanism of homologous recombination. Teaching Assistant in Biology, Anatomy and Physiology.

Master'S Degree, Cell/Cellular And Molecular Biology

Bachelor'S Degree, Biology/Biological Sciences, General