Project 1: Investigate the role of metal ions towards tau liquid-liquid phase separation.o First demonstration of metal induced liquid-liquid phase separation of intrinsically disordered tau proteino Developed new method for analysis for FRAP of small liquid protein droplets.o Responsible for design, implementation and management of multiple research projects; concurrently supervise and direct the research projects of one graduate student.o Review grant proposals and manuscript submissions. Project 2: Investigate the role of structural polymorphism of tau aggregates in Alzheimer’s disease (AD).o Developed method for purification of tau filaments from brain tissue of AD patients.o Developed and optimized method for Hydrogen/Deuterium exchange and Hydroxyl radical foot printing mass spectrometry of tau and its short variant.

Project: Huntington’s disease: Small molecule-based therapeutic strategies and metabolomics analysiso Discovered two small molecule inhibitors active against polyglutamine aggregation involved in HD.o Developed a strategy for fly head separation and metabolite extraction for NMR metabolomics.o Discovered altered energy metabolism in first metabolomics study using Drosophila HD model.o Published 3 first author publications in reputed American chemical society journals, filed 2 Indian patent applications and received best thesis award.a. Singh V, Patel KA, Sharma RK, Patil PR, Joshi AS, Parihar R, Athilingam T, Sinha N, Ganesh S, Sinha P, Roy I, Thakur AK (2019). Discovery of Arginine Ethyl Ester as Polyglutamine Aggregation Inhibitor: Conformational Transitioning of Huntingtin N-Terminus Augments Aggregation Suppression. ACS Chem Neurosci. 10(9):3969-3985.b. Singh V, Deepak RNVK, Sengupta B, Joshi AS, Fan H, Sen P, Thakur AK (2018). Calmidazolium chloride and its complex with serum albumin prevent Huntingtin exon1 aggregation. Mol Pharm. 15(8):3356-3368. c. Singh V, Sharma RK, Athilingam T, Sinha P, Sinha N, Thakur AK (2017). NMR spectroscopy-based metabolomics of Drosophila model of Huntington’s disease suggests altered cell energetics. J Proteome Res. 16(10):3863-3872.

A collaborative initiative of Department of Biological Sciences andBioengineering (BSBE), Design Innovation Centre, SIDBI Innovation andIncubation Centre (SIIC) and King George's Medical University (KGMU),Lucknow for IITK students.We as a team interacted with doctors to understand technology requirements that could improve the daily patient health management. We believe better communication, better instrumentation, better design (product or process) can significantly impact the healthcare system of India.o Designed portable cervical cancer screening endoscope (IITK-KGMU clinical immersion program).o Coordinated team of doctors and engineers to successful early stage funding of $70,000 from Biotechnology Ignition Grant Scheme (BIG) - BIRAC.

I will be responsible for internships and placements of BSBE department UG's and PG's.

Biochemical and Bio-Analytical Expertise (GLP lab) Handling the Inprocess analysis of Human Insulin and its analogs, r-trypsin, Protein-A (Bakers Bond), r-streptokinase, T1h and rhG-CSF. Size exclusion chromatography, Peptide mapping (HPLC), Particle size and Total organic Carbon (TOC) analysis, Isoelectric Focusing (IEF), SDS-PAGE. Cleaning Sample Validation and Human Insulin intermediate sample stability study. In-house reference establishment and Characterization using analytical and statistical tools.Handling of HPLC (Waters and AGILENT 1100 & 1200 Series with Empower, Chemstore and Ezchrome elite software) and AGILENT GC, Mastersizer, UV spectrophotometer, TOC Analyzer, Microfluidizer, Atomic Absorption spectroscopy (AAS).Establishment of systemsGLP compliant documentation.Designing and Validation of datasheets for various stages involved in Human Insulin Inprocess. Discrepancy management and training of employees.Development of new analysis method for Human Insulin Inprocess analysis.Developed and implemented new sample pooling method to reduce holding time by 30% during Insulin downstream processing.Method transfer for Insulin analogs from HPLC to UHPLC.Other responsibilitiesTechnology Transfer - Calibration of Balance (Sartorius and Mettler Toledo).Installation and validation of instruments used in Lab.Working in accordance with 21 CFR (Code of Federal Regulations) and FDA guidelines.Compilation of Annual Batch Trend (ABT).