Senior Research Fellow
Amrita Centre For Nanosciences & Molecular Medicine, Amrita Viswa Vidyapeetham
Kochi, Kerala, India
Carried out the project entitled "The development of metformin loaded polymeric nanoparticles for pancreatic cancer application"., funded by Dept. of Biotechnology, Govt of India. Work involved optimization of nanoparticle synthesis using different biodegradable polymers including Chitosan, PLGA, PLA, Dextran sulfate, and Eudragit for drug delivery applications by employing different methods. Expertise in physicochemical characterization techniques for determination of size, shape, drug… Show more Carried out the project entitled "The development of metformin loaded polymeric nanoparticles for pancreatic cancer application"., funded by Dept. of Biotechnology, Govt of India. Work involved optimization of nanoparticle synthesis using different biodegradable polymers including Chitosan, PLGA, PLA, Dextran sulfate, and Eudragit for drug delivery applications by employing different methods. Expertise in physicochemical characterization techniques for determination of size, shape, drug loading, and functional groups using DLS, SEM, TEM, Spectrophotometry, FT-IR respectively. Proficient in various in-vitro assays including, cell viability/cytotoxicity (MTT/Alamar Blue), colony formation (Microscopy), cell migration assay, cell cycle analysis, cell uptake assays (Flow cytometry, Confocal imaging, HPLC), apoptosis, and ROS generation assay (flow cytometry), gene expression (PCR). Optimized HPLC protocol for drug quantification (metformin) from tissues and plasma. Experience in in-vivo PKPD & ADME profiling of nanoformulations (Oral & IV), determination of combinatorial drug activity, biodistribution (in vivo NIR imaging) Co-supervised postgraduate students for dissertation work.Awarded Senior Research Fellowship (SRF) by CSIR, Govt of India. Show less