• Cell culture process development and technology transfer for biologic production• Experience in perfusion and fed-batch processes• Perfusion processes include ATF and microcarrier cultures• Successfully established and transferred client processes to cGMP production

Responsible for cell culture process development and technology transfer for biologic production. Experienced in perfusion and fed-batch processes. Perfusion processes include ATF and microcarrier cultures. Successfully transferred clients processes to cGMP manufacture.

Generated production cell lines. Process development for animal component free process with reduced timelines. Technology transfer to cell banking, RegTox and clinical supply production facilities. Cell banking process development for bacteria. Process development using liquid handlers.• Developed and successfully transferred an animal component free cell banking process for an anaerobic bacteria to a GMP facility in support of Pfizer’s Vaccine Research Unit• Generated production… Show more Generated production cell lines. Process development for animal component free process with reduced timelines. Technology transfer to cell banking, RegTox and clinical supply production facilities. Cell banking process development for bacteria. Process development using liquid handlers.• Developed and successfully transferred an animal component free cell banking process for an anaerobic bacteria to a GMP facility in support of Pfizer’s Vaccine Research Unit• Generated production cell lines for 4 projects and successfully transferred processes to cell banking, RegTox and clinical supply manufacturing facilities• Continual process improvements reduced timelines for generating CHO production cell lines from 72 weeks to less than 15 weeks reducing cost and improving efficiency• Trained colleagues in bioreactor operation and learned cell line generation processes resulting in improved group capability• Team evaluation of bioreactor control systems led to the selection of an improved development platform system that is scalable and adaptable• Designed a strategy to use a liquid handler in the cell line selection process allowing higher throughput and ensuring accurate data tracking • Laboratory operation team eliminated supply and equipment bottlenecks improving lab efficiency and reducing project timelines• Laboratory move teams minimized laboratory down time during moves from Creve Coeur to Chesterfield and from Chesterfield building BB to building HH and move planning resulted in lab design/equipment placement led to operation efficiency improvement Show less

Cell Line Development generated production cell lines. Process Development of in-house expression systems using NS0 and CHO cells.• Developed animal component free processes for NS0 and CHO expression systems eliminating FDA regulatory and purification issues• Modified enzymatic assays measuring cholesterol, glutamine and ammonia to 96-well plate format resulting in easy to use assays for in process monitoring of these nutrient/metabolites• Worked with design engineers and… Show more Cell Line Development generated production cell lines. Process Development of in-house expression systems using NS0 and CHO cells.• Developed animal component free processes for NS0 and CHO expression systems eliminating FDA regulatory and purification issues• Modified enzymatic assays measuring cholesterol, glutamine and ammonia to 96-well plate format resulting in easy to use assays for in process monitoring of these nutrient/metabolites• Worked with design engineers and architects to develop renovation plans to improve capacity and efficiency for cell culture labs in Creve Coeur which resulted in a decision not to renovate but to move operations to Chesterfield Show less

Produced mammalian cell derived proteins from large scale cell culture systems in support of Searle’s research projects. Updated and maintained cell culture equipment.• Met or exceeded production demands and timelines for mammalian expressed proteins using flasks, roller bottles, spinner flasks and bioreactors• Doubled production capacity by constructing bioreactor work stations and vessels • Improved a hybridoma medium formulation using HPLC amino acid analysis resulting in… Show more Produced mammalian cell derived proteins from large scale cell culture systems in support of Searle’s research projects. Updated and maintained cell culture equipment.• Met or exceeded production demands and timelines for mammalian expressed proteins using flasks, roller bottles, spinner flasks and bioreactors• Doubled production capacity by constructing bioreactor work stations and vessels • Improved a hybridoma medium formulation using HPLC amino acid analysis resulting in a robust batch production medium• Identified commercially available serum-free media for BHK cells eliminating purification issues• Updated a 1000 L per day purified water system resulting in improved reliability and less maintenance Show less

Supported research with human skin collagenase from normal and RDEB groups. Skills used included: Biochemistry, Biology, Cell and Molecular Biology, and equipment maintenance and repair.• Maintained critical cell banks and cell culture equipment used in the Dermatology research laboratories• Purified collagenase from normal and RDEB human fibroblasts needed for use in ELISA, activity assays, and rabbit polyclonal antibody production• Produced and purified rabbit polyclonal… Show more Supported research with human skin collagenase from normal and RDEB groups. Skills used included: Biochemistry, Biology, Cell and Molecular Biology, and equipment maintenance and repair.• Maintained critical cell banks and cell culture equipment used in the Dermatology research laboratories• Purified collagenase from normal and RDEB human fibroblasts needed for use in ELISA, activity assays, and rabbit polyclonal antibody production• Produced and purified rabbit polyclonal antibodies needed for ELISA and Western blot assays• Isolated and analyzed mRNA using cell free translation systems to identify the impact of various drugs on collagenase message production Show less