Stephanie Holley Email and Phone Number

Developed two kinase selectivity/specificity assays using Invitrogen Z' Lyte FRET platform for weekly in-house screening of novel small molecule inhibitors* Created a mobility-shift enzyme assay using Caliper-based microfluidic technology for a receptor tyrosine kinase with a specific amino acid mutation* Provided weekly IC50 results for kinase assays to support medicinal chemistry* Expressed recombinant His-tagged proteins of interest using baculovirus infection system with Sf9/Sf21 insect cells and bacterial E. coli system* Performed protein expression checks by immunoprecipitation and western blotting

Performed PCR testing of Lyme and Babesia pathogens in deer ticks* Identified ticks by species, sex, and life stage * Processed serum, blood, and cerebral spinal fluid specimens daily for testing

Responded to calls on HelpDesk providing support in PC/mainframe environments