Stephen Chambers Email & Phone Number

Naas

Currently a laboratory scientist within the microsatellite (MS) department at Weatherbys Scientific. Responsibilities: 1) Parental verification of equine samples using MS DNA. This is achieved via:• The extraction of DNA from hair and blood samples for thoroughbred and non-thoroughbred varieties of horse.• Amplification of MS DNA markers via polymerase chain reaction (PCR).• Size separation of MS DNA via capillary electrophoresis and subsequent analysis using Gene mapper… Show more Currently a laboratory scientist within the microsatellite (MS) department at Weatherbys Scientific. Responsibilities: 1) Parental verification of equine samples using MS DNA. This is achieved via:• The extraction of DNA from hair and blood samples for thoroughbred and non-thoroughbred varieties of horse.• Amplification of MS DNA markers via polymerase chain reaction (PCR).• Size separation of MS DNA via capillary electrophoresis and subsequent analysis using Gene mapper software.2) To determine the presence/absence of alleles responsible for hoof wall separation disease within Connemara ponies. This is achieved using a Real-time polymerase chain reaction assay (qPCR). 3) Quantify DNA samples after whole genome amplification prior to sequencing on the Illumina assay. Show less

Galway, Ireland

• Name of final year project: "The Isolation and Expression of a Recombinant Antibody Fragment for Prostate Cancer Targeting". • Undertaken/completed within the Recombinant protein lab, Microbiology department, National University of Ireland, Galway. • Supervision: My academic supervisor was Prof. Gerrard Wall, my laboratory supervisor was Delphine Antoine. • Description of project: A mature, fully humanized, phage display library was screened for the presence of an anti-PSMA… Show more • Name of final year project: "The Isolation and Expression of a Recombinant Antibody Fragment for Prostate Cancer Targeting". • Undertaken/completed within the Recombinant protein lab, Microbiology department, National University of Ireland, Galway. • Supervision: My academic supervisor was Prof. Gerrard Wall, my laboratory supervisor was Delphine Antoine. • Description of project: A mature, fully humanized, phage display library was screened for the presence of an anti-PSMA antibody. Once isolated, the genes conferring said antibody was transformed into E. coli cells for overexpression of the protein. • Laboratory Skills employed: Gel Electrophoresis, Polymerase chain reaction (PCR), Western blotting, Restriction digestion, Enzyme-linked immunosorbent assay (ELISA), DNA Transformation, Plasmid isolation. Show less

Bachelor'S Degree, Microbiology, General, 68.08% - 2.1.

Activities and Societies: 1) Member of the NUIG surf club. 2) Teaching assistant for second-year laboratory classes. 3) Class.

Secondary School, Cao Points: 445.

Optional subjects: Chemistry, Biology, Home economics, and Geography.