Sumera Perveen Email & Phone Number

Toronto, Ontario, Canada

Toronto, Ontario, Canada

Structural, biochemical and biophysical characterization of human and COVID proteins by using X-ray Crystallography, protein-protein interaction assays, binding assays, and thermal denaturation assays such as, Fluorescence polarization (FP) assays, Time-resolved fluorescence energy transfer (TR-FRET) assays, radiometric (RA) assays, Isothermal Titration Calorimetry (ITC), Surface Plasmon Resonance (SPR), Differential Scanning Fluorimetry (DSF) and Differential Static Light Scattering (DSLS) to support the discovery of chemical probes.

Waterloo, Ontario, Canada

In vitro refolding of recombinant tryptophan synthase pathway enzymes were carried out by different methods such as refolding and by implying different expression conditions. Proteins were denatured using urea (8 M) as denaturing agents and refolding by gradual removal of denaturing agents. For proper refolding of tryptophan synthase α and tryptophan synthase β, the tryptophan synthase enzymes were His tagged at N-terminal and were co-expressed with the GroEL4S to get the soluble and enzymatically active proteins.

Turku, Southwest Finland, Finland

Crystallographic studies of enzymes involved in tryptophan biosynthesis was done as part of my PhD thesis. Crystals were obtained for two proteins; crystal structure was solved which resulted in two publications.

Lahore, Punjab, Pakistan

Taught Biochemistry (Enzymology, Carbohydrate metabolism, Lipid metabolism, and Protein Biochemistry) to BS Psychology class of forty students and to BS (Hons) Biotechnology class comprising 50 students. Provided supervision in Biochemistry Lab and helped them in learning basic laboratory techniques.

Lahore, Punjab, Pakistan

Thesis title: Tryptophan biosynthesis pathway in hyperthermophilic archaeon, Thermococcus kodakarensis.Gene cloning, expression, purification and biochemical characterization of enzymes of Tryptophan biosynthesis pathway was done

Lahore, Punjab, Pakistan

M. Phil in Biological SciencesThesis: Gene cloning and expression, in Escherichia coli, of tryptophan synthase α- & β- subunit from hyperthermophilic archaeon Pyrobaculum calidifontis.Tryptophan synthase α- and β- subunit from Pyrobaculum calidifontis were amplified by PCR, cloned and expressed in Escherichia coli for further purification and characterization studies

Faisalabad, Punjab, Pakistan

MSc in BiochemistryThesis: In vitro interaction of citalopram with human plasma protein.Protein binding of citalopram was measured in vitro with human plasma proteins at therapeuticplasma levels. The binding of the drug to plasma was determined by using the ultrafiltration technique and High-Performance Liquid Chromatography.

Lahore, Punjab, Pakistan

Involved in Clinical Cytogenetic and Microbiology Labs with expertise in Cytogenetic techniques such as karyotyping, DNA extraction, setting up of peripheral blood cultures, harvesting the culture, making slides, staining, and analysis of human chromosomes. Carried out Clinical Microbiological procedures from microbiological clinical sample reception to reporting including culturing pathogens from different specimens, their identification, biochemical testing, and antimicrobial sensitivity testing.

Lahore, Punjab, Pakistan

Skilled in all Pathology labs (Microbiology, Hematology and Blood Banking , Clinical Pathology and Serology, Clinical Biochemistry, Histopathology with specialization in Exfoliative Cytology and Cytopathology with hands on experience in preparation and screening various cytological specimens which includes pap smears, various fluids and FNA’S, processing all tissue specimens (lungs, brain, muscle, eyes, GIT Specimens, frozen section by cryostat) from their fixation, dehydration, clearing, embedding, sectioning by microtomy up to staining. Staining clinical samples using staining techniques like haematoxyllin and eosin staining, PAS, alcian blue, osmium tetraoxide, masson fontana, von kossa’s and congo red.

Postdoctoral Fellowship

Doctor Of Philosophy - Phd, Protein Biochemistry

Doctor Of Philosophy - Phd, Structural Biology

Doctor Of Philosophy - Phd, Biological Sciences, Biochemistry And Molecular Biology, 95% Marks In Comprehensive Exam With Grade Point 4.0

Thesis title: Tryptophan biosynthesis pathway in hyperthermophilic archaeon, Thermococcus kodakarensis. Biochemical and structural.

Master Of Philosophy - Mphil, Biology/Biological Sciences, General, Grade Distinction With 86% Marks And A+ Grade (Cgpa: 3.82/4)

Thesis title: Gene cloning and expression, in Escherichia coli, of tryptophan synthase α- and β- subunit from hyperthermophilic archaeon.

Master Of Science - Ms, Biochemistry, Awarded University Merit Scholarship On Achieving Grade A (Cgpa: 3.79/4)

Thesis title: In vitro interaction of citalopram with human plasma protein.

Bachelor'S Degree, Clinical Laboratory Science/Medical Technology/Technologist

Curiosity to know about the clinical applications of basic science lead me to Medical Laboratory Technology where I gained practical.