Sylvia Choi Email & Phone Number

Emeryville, California, United States

Cell culture team (US)

Emeryville

MS&T Analytical • Trained personnel on laboratory equipment and on variant assays. • Reviewed and edited Pilot Plan Operational Procedures (PPOPs), Test Methods, Work Instructions, Tech Transfer Reports for assays, and Qualification Reports working with QC and QA. • Performed qualification assays along with providing analysis results for Certificate of Analysis (CofA). • Leads several projects including CDMO projects; supervised tech transfer from R&D to MS&T, training QC for… Show more MS&T Analytical • Trained personnel on laboratory equipment and on variant assays. • Reviewed and edited Pilot Plan Operational Procedures (PPOPs), Test Methods, Work Instructions, Tech Transfer Reports for assays, and Qualification Reports working with QC and QA. • Performed qualification assays along with providing analysis results for Certificate of Analysis (CofA). • Leads several projects including CDMO projects; supervised tech transfer from R&D to MS&T, training QC for tech transfer projects and many more• Responsibilities include performing assays, writing and reviewing documents including qualification reports, working with QC and QA to approve and make the documents effective• Organizes team meetings with other groups involved in the same projects and supports ongoing development studies• Performing and writing qualification for In-process and Final Products such as SDS-PAGE, SEC, Pierce, A280, Western, Mass Spec and many more Show less

Emeryville

MS&T Analytical • Actively involves in laboratory-based analytical testing, and support ongoing development studies & Manufacturing investigations in a timely manner• Leading in assay improvement, development & qualification projects with producing Certificate of Analysis• Develop assays for drug characterization and write qualification assays, delivering on time• Coordinating with cross functional team to help process development with the analytics and discuss the design of the… Show more MS&T Analytical • Actively involves in laboratory-based analytical testing, and support ongoing development studies & Manufacturing investigations in a timely manner• Leading in assay improvement, development & qualification projects with producing Certificate of Analysis• Develop assays for drug characterization and write qualification assays, delivering on time• Coordinating with cross functional team to help process development with the analytics and discuss the design of the next studies of antigen projects Show less

Berkeley, Ca

• Optimized conditions for lysing cells using nitrogen disruption to achieve better yield efficiency• Developed mitochondria purification method for Solanum tuberosum “Potato” (plant) • Cultured eukaryotic cells & purified and characterized membrane proteins for several research projects simultaneously • Achieved better yield by optimization the purification and high purity of purified proteins using AKTA gel filtration column for better structural analysis by cryo-EM (Electron… Show more • Optimized conditions for lysing cells using nitrogen disruption to achieve better yield efficiency• Developed mitochondria purification method for Solanum tuberosum “Potato” (plant) • Cultured eukaryotic cells & purified and characterized membrane proteins for several research projects simultaneously • Achieved better yield by optimization the purification and high purity of purified proteins using AKTA gel filtration column for better structural analysis by cryo-EM (Electron Microscopy)• Supervised five UC Berkeley undergraduates, training them in laboratory-based analytical testing & interpretation of results, managing them in the successful completion of their project goals each semester Show less

Urbana-Champaign, Illinois Area

• Research guidance under Prof. Robert B. Gennis.• Organized with other labs on campus (5) and outside universities (2) leading to 3 first author papers from well known journals: JACS, BBA, and Photochemistry and Photobiology.• Identified the enzyme substrate binding site by developing assays, specifically for quionone. Moreover, identified the mistakes of what was overlooked using Q1 as a substrate in assays people commonly use.• Research: Biochemical and biophysical studies on… Show more • Research guidance under Prof. Robert B. Gennis.• Organized with other labs on campus (5) and outside universities (2) leading to 3 first author papers from well known journals: JACS, BBA, and Photochemistry and Photobiology.• Identified the enzyme substrate binding site by developing assays, specifically for quionone. Moreover, identified the mistakes of what was overlooked using Q1 as a substrate in assays people commonly use.• Research: Biochemical and biophysical studies on membrane proteins from E. coli, specifically the quinol oxidase of its substrate binding and oxygen pathway Show less

Urbana

• Research guidance under Prof. Robert B. Gennis.• Actively coordinated collaborations with labs throughout the campus (5), domestic universities (2) and international universities (2) to conduct interdisciplinary research• Successfully delivered different projects on deadlines and produced 6 co-authored papers, including PNAS, Biochemistry and others• Conducted molecular biology experiments involving culturing cells (E. coli, Rhodobacter sphaeroides, Thermus thermophilus) and… Show more • Research guidance under Prof. Robert B. Gennis.• Actively coordinated collaborations with labs throughout the campus (5), domestic universities (2) and international universities (2) to conduct interdisciplinary research• Successfully delivered different projects on deadlines and produced 6 co-authored papers, including PNAS, Biochemistry and others• Conducted molecular biology experiments involving culturing cells (E. coli, Rhodobacter sphaeroides, Thermus thermophilus) and purifying and characterizing proteins for several research projects • Developed the enzymatic assay to study the transient/fixed nature of a substrate interaction with membrane protein which later on show a huge impact on how the field was neglecting the huge important role of the substrate tail interaction to the protein• Optimized purification of cytochrome bo3 membrane protein from E. coli (homologous expression) mutants with up to 3x yield and 99% purity for characterization of its function• Managed lab operations in maintaining equipment, assigning duties, supervising completion of responsibilities, equipment inventory and purchasing, and keeping within budget • Mentored and advised two undergraduates in biochemical techniques and trained them to be able to work independently and managing them in the successful completion of their project goals each semester• Managed to identify the proton loading site as well as proton pumping site, using bioinformatics (phylogenetic tree), molecular biology, structural identification, biochemical and biophysical techniques, which was unidentified before• Research: Biochemical and biophysical studies on heme copper oxygen reductase membrane proteins in respiratory chain Show less

Bioenergetics

• Organized and conducted discussion sessions for junior and senior level courses in physical biochemistry. (100 students/ class)• Evaluated reports, homework and exams.- Objectively assessed their work and mentored them to stay on track. (30 students/ class)• TA for Physiology Lab for senior students and looked into physiological phenomena using animal models.

Optoelectronics And Semiconductors Laboratory

• Participated in the cutting-edge science of semiconductors studying their optical properties using spectroscopy(Photoluminescence and resonance Raman spectroscopy).• Independently developed a research idea and actively collaborated with a national lab, KIST (S. Korea) to pursue project.• Study of ZnSe nanowires, carbon nanotubes, Quantum dots measuring its photoluminescence (PL) and Raman spectroscopy.• Techniques such as AFM and TEM was used to look at the structure of the… Show more • Participated in the cutting-edge science of semiconductors studying their optical properties using spectroscopy(Photoluminescence and resonance Raman spectroscopy).• Independently developed a research idea and actively collaborated with a national lab, KIST (S. Korea) to pursue project.• Study of ZnSe nanowires, carbon nanotubes, Quantum dots measuring its photoluminescence (PL) and Raman spectroscopy.• Techniques such as AFM and TEM was used to look at the structure of the samples. Show less

• Taught lab in Physics introductory course for freshmen. (30 students / class)• Mentored students, graded tests and reports to assess and evaluate their performance.• Organized and evaluated quizzes for each class.

Magnetic Materials Laboratory

Measuring the magnetic particle size

Doctor Of Philosophy (Phd), Center For Biophysics And Computational Biology

Master'S Degree, Applied Physics

Bs, Physics

Doctor Of Philosophy - Phd, Biochemistry/Biophysics