Analytical chemistry and medical research intern at iC42 LabDepartment of AnesthesiologyUniversity of Colorado Anschutz Medical CampusBioanalytical technologies- principles and hands-on use with focus on HPLC, GC, and mass spectrometry:• Development of validation of a GC-MS/MS assay for the terpene limonene• Analysis of study samples collected during a clinical trial from healthy participants• Set up of an assay to quantify Monoacylglycerinlipase (MAGL) activity in vitro and ex vivo (rat brain tissue)• In the context above, set up and validation of an LC-MS/MS assay to quantify endocannabinoids and arachidonic acids (unlabeled and isotope labeled)• Set up and execution of calibration and quality control strategies for said assay• MAGL activity studies and measurement of study samples• Data acquisition, integration of ion chromatograms and data analysis• Data interpretation in context of the literature• Sample preparation and analysis of Sirolimus in coronary tissues after deployment of drug-eluting balloons in a Yorkshire pig model• Quality control• Documentation as fit for a regulatory compliant study (21CFR Part 58)• Summary of data in EXCEL spreadsheet format• Study planning and design• Assay validation, trouble shooting and devising alternative strategies• In vitro enzyme binding studies• Use of key software packages (Sciex Analyst, Multiquant, GraphPad Prism)

• Planning, organizing and leading International Friends events hosted by Esslingen University of Applied Sciences • Tutoring international exchange students • Managing the social media of the International Office• Translating from and to Russian, English and German

Project cooperation between Exyte and Esslingen University of Applied SciencesParticipated in the development of the concept study for the following task:Based on a block flow diagram with a process description and a capacity specification, the team was to develop a concept for a plant for the biotechnological production of active ingredients in a mammalian cell culture. The protein purification process was considered to be available.The concept was to include the following services:• Setting up a project organization including a schedule for the planning, implementation and execution phasesProduction planning• Creation of process flow diagrams with information on material flows as well as cleaning and sterilization concepts• Dimensioning of main equipment and product lines• Quantity balance for raw materials and cleaning agents• Building layout with cleanroom zones, personnel and material flows and all necessary ancillary areas, e.g. for IPC laboratories, administration and others• Room list• Main equipment list• Procurement schedule• Ventilation concept• Determination of investment costs with a focus on the cost structure