Department of OncogenomicsSupervisor: William Faller, PhD→ Designed, optimized and implemented an innovative technique based on deep-sequencing of ribosome-protected mRNA fragments (Ribosome Profiling) in both animals and 3D organoid models. The success of this approach allowed for 10+ projects to be developed in the host group and external ones.→ Awarded with an EMBO Long-Term Fellowship (success rate 15%) to fund my research. Planned, executed and analyzed experiments that helped secure funding of 2x100.000€ (CGC) and 350.000€ (NWO).→ Strengthened oral communication skills by presenting findings in 7 international conferences to audiences of 200+, and updating project progress regularly to audiences ranging from 5-50.→ Co-authored 6 peer-reviewed scientific articles published in high ranked journals (ex: Nat Comms, IF=18 and NAR, IF=19)→ Contributed to the development of 2 new bioinformatic tools (RiboODDR and dripARF) with experiments and analytical input.→ Co-supervised MSc and BSc students🧬 Relavent techniques:• Mouse models• Organoid culture• Cell culture• Ribosome profiling (in vitro and in vivo)• Mitoribosome profiling (in vitro and in vivo)• RNA sequencing• Immunoprecipitations and proximity assays• Protein synthesis• DNA/RNA/protein extraction from cells, organoids and tissues• Gel electrophoresis• qRT-PCR• Western blot• Seahorse and other live cell metabolic assays• Immunohistochemistry📌 Courses:• Project Managing for Scientists• Applying Design Principles to Schematic Figures• Manuscript and Grant writing• Animal experimentation (Art #9)

Department of OncologySupervisor: Travis Stracker, PhDCum Laude→ Developed problem-solving, critical thinking and hypothesis-driven approaches by designing, optimizing and executing multiple experiments that were outside the expertise of the host research group.→ Set up collaborations and travelled to external institutes to learn techniques that I later implemented in the host institute.→ Led my thesis entitled "Functional analysis of EXD2 in mitochondrial homeostasis", which was successfully published in Nat. Cell. Biol. (IF=28). The article was chosen as the cover story and a "News&Views" piece was written about it.→ Awarded with a FCT PhD scholarship (success rate 20%) to fund entirely my PhD studies→ Developed both oral and written skills by presenting my PhD project in 8 scientific conferences with audiences of 150-300, monthly work updates for audiences of 10-60 and writing annual scientific reports.🧬 Relavent techniques:• Xenografts• Drosophila melanogaster• Cell culture• DNA/RNA/protein extraction from cells, blood and tissues• Gel electrophoresis• qRT-PCR• Western blot• Immunofluorescence (confocal)• Immunohistochemistry• Seahorse and other live cell metabolic assays• FACS📌 Courses:• Animal experimentation• Scientific communication (oral and written)

Department of Virology and Molecular OncologySupervisor: Rui Medeiros, PhD→ Involved in multiple research projects investigating new biomarkers for prostate and colorectal cancers.→ Awarded with a full-time fellowship from the Portuguese League Against Cancer to support my research.→ Co-authored 5 peer-review scientific articles published in international journals. 🧬 Relavent techniques:• DNA/RNA extraction from blood, urine and tissue samples• PCR/RFLP• Gel electrophoresis• RT-qPCR