Thien Vu Email & Phone Number

Boston, MA, US

Led cross-functional efforts to produce and characterize over a trillion neuronal cells, delivering the world's first phenomic neuronal map that revolutionizes our understanding of neurobiology. Implemented data analytics tools to integrate diverse biological modalities in cell context data packages and rapidly develop scalable methods for deriving.

Led cross-functional team efforts that enabled Recursion to become one of the largest producers of high-quality human pluripotent stem cells (hPSCs) derived neurons. Developed and implemented robust assays and data analyses to drive process optimization for hPSC-derived neuron production at scale.

Salt Lake City, Utah, United States

• Project: Investigating functions of synaptic proteins in induced neurons.In this project, I use CRISPR to engineer stem cells, convert them into neurons, and assay synaptic function using fluorescent reporters.
• Established stem cell culture practices.
• Developed stem cell lines for rapid conversion into neurons.
• Co-authored a funded grant to acquire a cell sorter Sony MA900 to facilitate genome editing, and establish novel stem cell lines.
• Developing high throughput methods for genome engineering in mouse stem cells (CRISPR and prime editing).
• Developing functional assay of synapses of induced neurons.

Salt Lake City, Utah, United States

• Project: Regulation of vesicle docking, and vesicle recycling at the presynaptic terminals.
• Visualized vesicle docking, vesicle fusion, and vesicle recycling at murine presynaptic terminals using zap-and-freeze electron microscopy. Zap-and-freeze triggers an action potential in neurons with a short electrical.
• Characterized defects in synaptic mutants or with pharmacological disruptions using electron microscopy.
• Developed and optimized methods for super-resolution imaging of proteins in living murine synapses.
• Improved methods to localize proteins at nanoscopic resolution in electron micrographs.
• Optimized methods for primary neuron culture, and lentiviral production.

Woods Hole, Massachusetts, United States

• Provided hands-on training, and facilitated experiment designs in advanced projects using electron microscopy.
• Mentored graduate and postdoctoral researchers in sample preparation (high-pressure freezing, freeze substitution), imaging (transmission and scanning electron microscopy), and quantitative image analysis (ImageJ and.

Tacoma, Washington, United States

• Project: Nucleoside Analog Synthesis Mentor: Dr. John Hanson, Chemistry. Optimized Dess-Martin oxidation in the synthesis of nucleoside.
• Project: Induced Gamma Activity in 3D Object Recognition. Mentor: Dr. David Andresen, Psychology. Bangs-Collier Scholar award Designed and performed EEG recordings from human subjects while they perceived 3D objects.
• Project: Computational modeling of the interactions between sugars and Bdellovibrio bacteriovorus's MalA. Mentor: Dr. John Hanson & Dr. Jeff Grinstead, Chemistry. Performed homology modeling for the MalA structure, and.

Phd, Biology

• In addition to being a graduate research assistant, I was a teaching assistant in graduate courses (Genetics and Genome, Protein and.

Bachelor Of Science - Bs, Biochemistry, Psychology