Vanessa Ji Email and Phone Number

• Conduct PCR, performing molecular cloning via Gibson Assembly.• Transform E. Coli for in vivo DNA replication, and implement colony PCR. Run gel electrophoresis to check DNA sizes and choose optimal clones accordingly.• Implement Miniprep to extract DNA from E. Coli by alkaline lysis, protein denaturation and DNA elution.• Conduct Yarrowia Lithium Acetate Transformation for optimal production of naringenin derivatives. Perform electroporation for DNA introduction to yeast.• Use Geneious Prime for plasmid map construction and primer picking.

• Fabricate cell membrane coated PLGA nanoparticles via single emulsion and sonication. Characterize nanoparticles with different methods.• Apply click chemistry to link algae and membrane coated nanoparticles to enhance antibiotic retention or TTX neutralization. Quantify and improve nanoparticle drug loading.• Use fluorescence microscopy to check success rate of click linking.• Model algal strain 125, 354, 1545, nanoparticles, cell membrane and aqua environment with 3D modeling software Blender and Chimera-X.

• In preparation for interdepartmental audit, inspected the validity of 40+ equipment, 30+ types of reagents, 10+ Standard Operation Procedures (SOPs) and 60+ experiment records on lab logbooks in the R&D lab.• In compliance with regulations during the market approval process of Deruiyi® (FSMP), updated records for 140+ expired and renewed API quality standards and product stability testing records. Updated 150+ reference substances in the warehouse database, examined 100+ records of official and working reference substances.• Complied with globalization requirements for local documentation, translated and reviewed 40+ local SOPs and workflows, topics covering validation and verification of analytical methods and equipment maintenance.