Wei Wei Email & Phone Number

Vancouver, Bc, Ca

Vancouver, British Columbia, Canada

Vancouver, Bc

675 West 10Th Ave, Vancouver

• Research laboratory: Dr. Pamela Hoodless Lab• Projects:1) Epigenetic modifications regulating hepatocellular carcinoma and hepatocyte differentiation; 2) Genome-wide analysis of miRNA and mRNA expression during liver development; 3) MiRNA functions during early liver development through mESC differentiation; 4) MiRNA functions in reprogramming embryonic fibroblasts into hepatocytes; • Collaboration: 1) Transcriptional regulation of novel midgut marker Nepn;… Show more • Research laboratory: Dr. Pamela Hoodless Lab• Projects:1) Epigenetic modifications regulating hepatocellular carcinoma and hepatocyte differentiation; 2) Genome-wide analysis of miRNA and mRNA expression during liver development; 3) MiRNA functions during early liver development through mESC differentiation; 4) MiRNA functions in reprogramming embryonic fibroblasts into hepatocytes; • Collaboration: 1) Transcriptional regulation of novel midgut marker Nepn; 2) Exploring the functions of cis-regulatory elements of MEIS1 using an HM-1 targeting and differentiation system. • Contributions: 1) Set up bioinformatic methods to study miRNA expression profiling; 2) Developed methods to validate miRNA-target pairs and EMT assay; 3) Optimized gene delivery through lentiviral vectors; 4) Developed methods for mESC differentiation into hepatoblasts; 5) Optimized HM-1 targeting protocol; 6) Developed protocol to direct the differentiation of HM-1 into mesendoderm cells; 7) Developing protocol to reprogram embryonic fibroblasts into hepatocytes; 8) Reviewed journal articles and grants; 9) Supervising co-op students.• Collaboration: 1) Transcriptional regulation of novel midgut marker Nepn; 2) Exploring the functions of cis-regulatory elements of MEIS1 using an HM-1 targeting and differentiation system. • Selected publications: 1) Wei W, Hou J, Alder O, Ye X, Lee S, Cullum R, Chu A, Zhao Y, Warner SM, Knight DA, Yang D, Jones SJ, Marra MA, Hoodless PA. Genome-wide microRNA and mRNA profiling in mouse liver development implicates mir302b and mir20a in repressing TGFbeta signalling. Hepatology, Hepatology. 2013 Jun;57(6):2491-501. IF: 12.0032) Xiang P, Wei W, Lo C, Rosten P, Hou J, Hoodless PA, Bilenky M, Bonifer C, Cockerill PN, Kirkpatrick A, Gottgens B, Hirst M, Humphries KR. Delineating MEIS1 cis-regulatory elements active in hematopoietic cells. Leukemia. 2013 Oct 7. doi: 10.1038/leu.2013.287. IF: 10.164 Show less

• Research laboratory: Dr. Hongkui Deng Lab• Projects: 1) Establishment of mouse, rat and human embryonic stem cells using small molecules; 2) Differentiation embryonic stem cells into germ line cells.• Contributions: 1) Developed a novel strategy with increased efficiency and ease of operation for mouse embryonic stem (ES) cell derivation; 2) Developed the first in vitro primordial germ cell (PGC) specification model with a chemically-defined culture system;… Show more • Research laboratory: Dr. Hongkui Deng Lab• Projects: 1) Establishment of mouse, rat and human embryonic stem cells using small molecules; 2) Differentiation embryonic stem cells into germ line cells.• Contributions: 1) Developed a novel strategy with increased efficiency and ease of operation for mouse embryonic stem (ES) cell derivation; 2) Developed the first in vitro primordial germ cell (PGC) specification model with a chemically-defined culture system; 3) Initiated the screening of small molecules for rat ES cell culture conditions by generating induced pluripotent stem (iPS) cells instead of traditional embryo culture method.• Contributions as group leader: 1) Arrangement of subprojects within group members; 2) Organized journal club and seminar; 3) Trained co-op students and technicians. Fostered effective communication and productive cooperation within a team of 20 researchers; 4) Wrote grants for Bill & Melinda Gates Foundation; 5) Reviewed manuscripts and grants.• Selected publications: 1) Shen Y*, Shi C*, Wei W*, Yu W, Li W, Yang Y, Xu J, Ying W, Sui X, Fang L, Li W, Yang H, Shen H, Ding S, Shi Y, Deng H. The heterogeneity and dynamic equilibrium of rat embryonic stem cells. Cell Res. 2011 Jul;21(7):1143-7 (* co-1st author). IF: 10.5262) Yang W*, Wei W*, Shi C*, Zhu Jin, Ying W, Shen Y, Ye X, Fang L, Duo S, Che J, Shen H, Ding S, Deng H. Pluripotin combined with LIF greatly promotes the derivation of ES cell lines from refractory strains. Stem Cells. 2009 Feb;27(2):383-9 (* co-1st author). IF: 7.7013) Li W, Wei W, Zhu J, Shi Y, Lin T, Hao E, Hayek A, Deng H, Ding S. Generation of rat and human pluripotent stem cells with mouse embryonic stem cell characteristics by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell. 2009 Jan 9;4(1):16-9. IF: 25.315 Show less

• Research laboratory: Dr. Hongkui Deng Lab• Project: Establishment of mouse model with mouse embryonic stem cells. • Contribution: Generated the first cloned mouse in China. • Academic activities: Participating in the translation of English biology textbook to assist the teaching of undergraduate students.• Selected publications:1) Li X, Wei W, Yong J, Jia Q, Yu Y, Di K. The genetic heterozygosity and fitness of tetraploid embryos and embryonic stem cells are crucial… Show more • Research laboratory: Dr. Hongkui Deng Lab• Project: Establishment of mouse model with mouse embryonic stem cells. • Contribution: Generated the first cloned mouse in China. • Academic activities: Participating in the translation of English biology textbook to assist the teaching of undergraduate students.• Selected publications:1) Li X, Wei W, Yong J, Jia Q, Yu Y, Di K. The genetic heterozygosity and fitness of tetraploid embryos and embryonic stem cells are crucial parameters influencing survival of mice derived from embryonic stem cells by tetraploid embryo aggregation. Reproduction. 2005 Jul; 130(1):53-9. IF: 3.5552) Li X, Yu Y, Wei W, Yong J, Yang J, You J, Xiong X, Qing T, Deng H. Simple and efficient production of mice derived from embryonic stem cells aggregated with tetraploid embryos. Mol Reprod Dev. 2005 Jun;71 (2):154-8. IF: 2.379 Show less

Phd, Stem Cell Differentiation And Proliferation

Thesis: Primordial germ cell specification from embryonic stem cells

Bsc, Veterinary Medicine

Activities and Societies: Minister in Practice in Student Union of Collage of Veterinary Medicine in China Agricultural University..