Widler Casy, Ph.D. Email & Phone Number

Boston, Ma, Us

Gainesville, Florida, United States

• Develop and optimize bioanalytical assays for protein detection, vector DNA quantification, and vector RNA transcript quantification.• Lead the establishment and implementation of a comprehensive infrastructure for tissue processing, ensuring high-quality sample preparation and analysis.• Process hundreds of animal tissues, including mouse and non-human primate tissues, for vector DNA quantification, vector RNA quantification, and protein detection.• Collaborate with contract research organizations (CROs) to design and oversee animal studies, ensuring timely and accurate data generation.• Assist in establishing strategic partnerships with academic principal investigators (PIs), fostering collaborations and leveraging their expertise.• Generate intellectual property through innovative technology development, contributing to the company's portfolio and assets.• Manage proof of ideas and proof of concept studies for pipeline program development, collaborating closely with program managers.• Work with program managers to create compelling slide decks for companywide and executive board meetings, effectively communicating project progress and results.• Build and maintain relationships with contract research organizations (CROs), contract development and manufacturing organizations (CDMOs), and external consultants to leverage specialized expertise and resources.

Gainesville, Florida, United States

Gene Therapy - R&DOur team team is developing gene therapy drugs to treat people who suffer from genetic diseases. Visit us at https://www.aavantibio.com/ to learn more about what we do, how we do it, and of course why we do what we do.

Dallas, Texas

Gene Therapy• Our laboratory uses adeno-associated viruses (AAV) as gene delivery vectors to target a wide range of rare genetic diseases which include Giant Axonal Neuropathy (GAN), Batten disease, Rett syndrome, Pitt-Hopkins syndrome, and others • My primary research focus is to develop novel AAV vectors that can cross the blood brain barrier and target different areas of the brain with high specificity and low toxicity to peripheral organs• Approaches that I am using to engineer these novel AAV vectors include:o Rational design o capsid shuffling• Facets of my projects also involve clinical trial phases

• Investigated the mechanism of protein synthesis in bacteria using cutting-edge single molecule biophysics methods and technology• Designed and executed two major projects to completion • Collaborated with internal and external research groups• Received grant writing training and wrote several research proposals• Served as consultant in grant writing with collaborators• Generated data for grants and fellowship applications• Acquired technical skills such as:o Protein purification (Affinity chromatography, Anion exchange chromatography, and Cation exchange chromatography) o Immunocytochemistry o Binding assay (Protein–DNA interaction, protein–protein interaction, protein-RNA interaction, DNA-RNA interaction)• Carried out various microbiological assays, and performed mammalian cell culture, bacterial cell culture, gene knockout, polymerase chain reaction, run-off transcription, subcloning, electroporation and other basic biochemical and molecular biology methods• Scrutinized and modified existing protocols to increase efficacy and efficiency• Developed new protocol for single molecule studies• Assessment of protein and reagent stability• Informed principal investigator of laboratory related concerns• Trained new graduate student and undergraduate student interns on how to use various instruments • Wrote and kept track of progress reports• Oversaw and conducted routine inspection of laboratory equipment for proper functionality• Represented the University of Missouri at Annual Biomedical Research Conference for Minority Students (ABCRMS) for 2 years (Mizzou ambassador for minority student recruitment)

Saint Charles, Missouri

• Performed independent study of soil strata at The Historic Daniel Boone Home in St Charles Missouri• Carried out plant pathology based assay• Contributed to the design and construction of a compost pile system to convert waste from the greenhouse into natural fertilizers• Coordinated undergraduate’s general biology and advance plant biology laboratory courses by providing various plant species upon request and helping with students’ hands experiences• Recorded and maintained an up-to-date inventory of greenhouse and laboratory supplies• Trained and supervised new student-workers• Orchestrated spring plant sale • Lead marketing campaign to increase the greenhouse customer base • Generated steady growth in plants sales • Managed greenhouse communication• Provided excellent customer service via email, phone, and in-person

Lansing, Michigan Area

• Participated in the 2012 Summer Research Opportunity Program (SROP) at Michigan State University in the Pharmacology and Toxicology Department• Investigated the role of enterochromaffin cells in visceral hypersensitivity and in gastrointestinal track diseases such as ulcerative colitis and irritable bowel syndrome• Operated a microtome (Cryostat) to generate thin cross section of rats’ intestines • Performed immunohistochemistry using thin cross-section of intestinal tissues derived from rats• Received training on data analysis (ANOVA, Chi-square test, Student's t-test …) using various statistical software which include R, SAS, and SPSS • Attended numerous workshops tailored for scientific writing, science communication/public speaking, and leadership development

Doctor Of Philosophy - Phd, Biochemistry

Bachelor Of Science - Bs, Chemistry With An Emphasis In Biochemistry, Cum Laude

Activities and Societies: Alpha Chi Honor Society