As biotechnician in the Biomedical MR Imaging & Spectroscopy Group i performed surgeries to induce stroke in multiple models i.e.- MCAo (middle cerebral artery occlusion) in rats and mice using a intraluminal thread to occlude the artery. - The thrombotic occlusion model in rats, where a blood clot is injected. - Thrombo-embolic occlusion in mice, where thrombin is injected directly in the MCA. - Electro-coagulation of the MCA in mice, in which the MCA is occluded using bipolar diathermy- Photothrombotic model in rats. In which light sensitive Bengal Rose is injected. Subsequently when a specific part of the skull is iluminated, Bengal Rose forms a thrombus.I acquainted myself with other techniques for example - Subarachnoid hemorrhage in rats, where you puncture the MCA. - Cerebral aneurysms in rats, bilateral ligation of posterior renal and left common carotid artery to induce hypertension. More basic techniques: intubation for mechanical ventilation, cannulation of the tail vein and catheterization of the femoral artery in ratsBehavioral tests: cylinder, beam walk, NDS (neurological deficiency scale), open field, corner turn, adhesive removal test.Physiology monitoring when rats are in the MRI scanner. Performing anatomical and functional scans on both 4,7 an 9,4 Tesla MRI scanners (Varian)

I have performed surgery and behavioral tests as indication of lesion size in a experimental stroke model in rats and mice.

With a intratracheal injection i have injected a mixture of technetium and small cell lung cancer cells. Using SPECT-CT the injection site was confirmed, after which the growth was monitored using IVIS and CT.