Yan Cao Email and Phone Number

• Established fluorescence-based second messenger detection, including calcium mobilization assay on FLIPR and cyclic AMP assay on Envision, delivered high quality data for four SAR projects• Developed biochemical kinase assays using absorbance measurement on spetramax340 for two discovery programs with diabetes indication• Generated several stable cell lines by sub-cloning GPCR receptors, stable expressing in mammalian cells and detecting with calcium assay on FLIPR• Identified lead compound by using random High Throughput Screening (HTS) of 150,000 compounds with β-arrestin assay• Discovered receptor antagonists using HTRF technology in a screening with 150,000 compounds• Collected and analyzed data, and compared the results with clients’ in-house data, EC50/IC50 of reference compound was always controlled within 3 folds • Increased the throughput of compound screening through optimizing the liquid handling procedure• Composed daily summaries and measurement protocols to describe the methodology used and the results obtained during assays• Reviewed reference books, and academic journal articles to set up a new assay and learn about recent developments• Involved in teleconferences with our clients on a regular basis• Troubleshoot by conducting parallel assays including temperature and pH of reagent test• Optimized assays by performing titration experiment to find out best condition

• Conducted mammalian cell culture and optimized transient transfection by tittering ratio between cell density and transfection reagent• Constructed DNA-based shRNA expression vectors driven by human U6 promoter• Characterized the efficiency of shRNA expressed by DNA vectors using RT-PCR and western blot, selected fragments which have more than 70% interference effect • Summarized and analyzed data, wrote experiment reports and communicated with clients through e-mails or teleconferences