Yan Pan Email & Phone Number

Baltimore, Maryland Area

Involved in studies on adult and pediatric traumatic and ischemic brain injury, mitochondrial bioenergetics, oxidative stress, apoptosis, and neuroprotection; provided technical support and data analysis as a Database Analyst/ETL Developer/Reporting Engineer ,load large amount of data into database tables using mssql and oracle, 3years data reporting and analyzing experience with large volume of data by using Oracle,SQL, access, Excel etc; generated financial and medical report to director and researchers; involved in technician and student trainings; participated in lab management and maintenance.

Involved in studies on mitochondrial function, oxidative stress and excitotoxic cell death; also involved in the study on the contribution of neuronal/glial apoptosis and necrosis as cell death pathways in animal (including transgenic mice) models of acute and progressive neurodegeneration; participated in lab management and maintenance.Molecular biology and biochemistry: Genomic DNA, RNA extraction and mRNA isolation; DNA cloning and sequencing; PCR; RT-PCR; single cell PCR; gene expression in prokaryotic and eukaryotic system; electrophoresis technique.Transgenic technique:Preparing sterile stud male mice and surrogate female mice; recovering fertilized eggs; microinjection technique (mice and fruit fly); transplanting embryo technique; maintaining of transgenic mouse lines and genotyping.Immunohistochemistry (Immunocytochemistry):Perfusion of rats and mice; dissection of fruit fly and rats/mice brain and spinal cord; preparing sections from fruit fly eyes and rats/mice brain and spinal cord by Microtome and Cryostat; Vibratome sectioning; immunohistochemistry staining; immunocytochemistry staining; Cresyl Violet staining of the nerve cells; TUNEL staining; immunofluorescence; FJB staining and counter staining.Electron microscopy.etc: ImmunoEM sectioning; tissue EM processing; thick, thin, semi-thin sectioning; toluidine blue staining; hematoxylin and eosin staining; silver cholinesterase ICC staining. Surgery of rats and mice; basic technique in pathology and physiology; keeping fruit fly stocks; primary cell culture; stem cell culture and transfection; protein assay and western blotting; immunoprecipitation; Stereo Investigator quantitation; mouse behavioral test.