Yoonju P. Email & Phone Number

San Francisco, California, United States

Executed signaling assays to study individual ligands as well as the synergy between ligands to identify the mechanism of action of CXCR4-GPCRx heteromers in endogenous cell-based systemDeveloped and optimized in vitro cell-based assays with AML and breast cancer cell lines to validate therapeutic agent’s ability to activate or inhibit CXCR4 Isolated human immune cells for further use in immunoassays to evaluate immunological impact of oncology-related therapeutic agents on… Show more Executed signaling assays to study individual ligands as well as the synergy between ligands to identify the mechanism of action of CXCR4-GPCRx heteromers in endogenous cell-based systemDeveloped and optimized in vitro cell-based assays with AML and breast cancer cell lines to validate therapeutic agent’s ability to activate or inhibit CXCR4 Isolated human immune cells for further use in immunoassays to evaluate immunological impact of oncology-related therapeutic agents on primary immune cellsDetermined protein expression and performed binding assays for antibodies to AML cell lines, human platelets, and human bone marrow CD34+ cells to assess differential binding between cancer cell lines and toxicity-relevant normal cell types via flow cytometry (Cytek Aurora)Organized results and data, analyzed, and presented findings in cross-functional meetings Show less

San Carlos, California, United States

Extracted DNA from blood and dissected tissue samples to help determine the presence of various diseases and genetic disordersMonitored daily quality control, calibration, and maintenance of various liquid handlers (Tecan) in order to effectively isolate plasmid, extract DNA, and normalize samples for further testing via NGS down the line

Pittsburgh, Pennsylvania, United States

Experimental Biochemistry (9 hrs/week) -- Jan. 2021-May 2021Guided upper-class students through experimental methods commonly used in biological research such as enzyme kinetics, UV spectroscopy, and isolation and quantification of biological molecules Worked closely with instructors to identify difficulties students were experiencing and to develop solutions--Frontiers, Analysis, and Discovery in Biological Sciences (FADS) (9 hrs/week) -- Aug. 2020-May… Show more Experimental Biochemistry (9 hrs/week) -- Jan. 2021-May 2021Guided upper-class students through experimental methods commonly used in biological research such as enzyme kinetics, UV spectroscopy, and isolation and quantification of biological molecules Worked closely with instructors to identify difficulties students were experiencing and to develop solutions--Frontiers, Analysis, and Discovery in Biological Sciences (FADS) (9 hrs/week) -- Aug. 2020-May 2021Mentored freshmen students through their semester long project of exploring how DNA mutations can affect protein expression in bacteriaCoordinated a few lectures introducing key lab concepts and techniques to the students before they began their experiments Show less

Woolford Laboratory

Studied the role of the Nsa1 group of four proteins (mak16, rpf1, rrp1, and nsa1) that enter the ribosomal assembly pathway together but leave at different times through genetic engineeringPerformed PCR, TAP tagging, and silver staining to answer whether Nsa1 proteins are dependent on each other to enter or exit the pre-ribosome

Greater Pittsburgh Area

Designed and executed a study using wearables to track physiological patterns of addiction patients undergoing recovery and assess their effects on cravings to develop a relapse prevention method Operated alongside National Institute on Drug Abuse (NIDA), insurance companies, and rehab centers to gauge interest in a proposed product and uncover common issues in the addiction therapy industry