Zachary Johnson Email & Phone Number

United States

Mississauga, Ontario, Canada

• Reporting to the Director of Research and Development, independently led development, optimization, and validation of a portfolio immunoassay, with ultimate goal of meeting regulatory specifications and FDA.
• Conducted in-depth literature reviews to investigate biomarker structure, clinical utility, matrix effects, and applicability in patient sub-populations.
• Developed sandwich immunoassays compatible with the Vital Bio platform from standard ELISA, leading the entire process from conception to implementation, including addressing challenges related to adaptation of a.
• Engaged extensively with regulatory standards, including predicate assay benchmarks, patents, and CLIA guidelines, to develop comprehensive metrics for designing an assay suitable for FDA approval.
• Delivered detailed data presentations and project updates to interdisciplinary and leadership teams regularly, enabling transparency and visibility to the development process and guiding subsequent stages of development.
• Collaborated on a wide-range of projects across R&D, providing support to teams and individuals in process optimization, and assay development and validation.

Cambridge, Massachusetts

• Under the guidance of Principal Investigator, conducted NGS and CRISPR experiments to elucidate novel genomic and proteomic characteristics of P. falciparum and its vectors within malaria-endemic regions (South America.
• Development, validation, and optimization of sensitive and robust NGS assays to inform infection status and novel haplotypes.
• New method development including implementation of synthetic DNA spike-ins to detect contamination during Illumina AmpSeq protocols.
• Create novel parasite lines using CRISPR, including validation and optimization.
• Conducted extensive literature reviews to support NGS assay development, method optimization, and data analysis.
• Presented intra- and inter-lab research updates, as well as presentations at cross-departmental meetings.

Boston, Massachusetts

Neafsey Lab at HSPH

Cambridge, MA

• As one of two research scientists leading panel development and production for multiple FirePlex immunoassay platforms, worked closely and collaboratively with New Product Development and R&D Scientists, as well as.
• Development of catalog and custom immunoassay biomarker panels across broad research areas, including neurodegeneration, immuno-oncology, inflammation, and drug-induced organ injury.
• Conducted experiments to assess multiplex immunoassay performance metrics, including: sensitivity ofdetection, dynamic range determination, antibody cross-reactivity, and biological linearity in multiplecomplex matrices.
• Optimization of immunoassay workflows to continually improve performance specifications.
• Training of newly hired Senior Scientist in FirePlex immunoassay panel development and productionprocesses.
• Created and validated quality control methods and metrics to support immunoassay panel development.

Greater Boston Area

Undergraduate Thesis: Evaluating Fidelity of DNA Polymerase using Human Inorganic Pyrophosphatase as a ModelGreen Chemistry Project included the synthesis of fluoridated, organic products using Selectfluor, using product purification techniques such as: HPLC-MS, GC-FID.

Cambridge, MA

-Process incoming and outgoing product-Aliquot antibodies for the purpose of storage and sale -Handle biochemicals, antibodies, and assay kits for domestic and international shipping-Perform quality assurance work on product

Bachelor'S Degree, Biochemistry

Activities and Societies: Green Chemistry Lab

Bachelor'S Degree, Psychology

Bachelor'S Degree, Psychology

Bachelor Of Science, Psychology