Genome Technology Access Center @MGI

The Washington University Genome Technology Access Center (GTAC@MGI) provides microarray, Q-PCR, and next-generation sequencing (NGS) services to the Washington University community as well as outside entities. The facility is CAP/CLIA certified for the handling of medical samples. The NGS GTAC team involves itself in every step of sequencing research, including experimental design, DNA/RNA QC, library construction, sequencing, and analysis of next-generation sequence data. The NGS core is equipped with four Illumina HiSeq 2000 sequencers and one MiSeq. The facility currently prepares Illumina sequencing libraries for RNA-seq, ChIP-seq, targeted capture, whole exome capture and genomic sequencing. For DNA QC, the team utilizes a Qubit fluorometer and an Agilent 2100 BioAnalyzer. Library contrusction is automated using an Agilent Bravo and a SPRIworks Fragment Library System. The GTAC provides its customers with raw sequence data and comprehensive bioinformatic analysis for RNA-seq, ChIP-seq, targeted capture, whole exome capture and genomic sequencing using extensively validated workflows. The microarray facility is currently staffed by six scientists. The Facility involves itself in every step of microarray research, including custom array design, array printing, QC and labeling of both DNA and RNA, microarray scanning, statistical analysis. We utilize all commercial formats, including Affy, Agilent, Illumina and Nimblegen.