Joshua Abbott Email & Phone Number

Washington Dc-Baltimore Area

Washington Dc-Baltimore Area

Rockville, Maryland, United States

• Biomarker and drug target discovery using RNA-seq and proteomics of patient derived tissue models.• Performed drug target validation using siRNA, shRNA, RNA-seq, and proteomic profiling to characterize disease mechanisms. Validation of biomarkers using qPCR and western blotting.• High-content analysis of neural stem cells, iPSCs, and brain organoids to characterize and validate drug targets and biomarkers.• Designed antisense oligonucleotides and evaluated their efficacy for exon… Show more • Biomarker and drug target discovery using RNA-seq and proteomics of patient derived tissue models.• Performed drug target validation using siRNA, shRNA, RNA-seq, and proteomic profiling to characterize disease mechanisms. Validation of biomarkers using qPCR and western blotting.• High-content analysis of neural stem cells, iPSCs, and brain organoids to characterize and validate drug targets and biomarkers.• Designed antisense oligonucleotides and evaluated their efficacy for exon skipping in in vitro models.• Developed bioinformatics analysis pipelines for RNA-seq and global proteomic profiling analysis using R.• Utilized CRISPR/CAS9 in patient derived iPSCs characterize disease mechanisms. Show less

Greater Denver Area

• Developed in vitro assays to characterize inhibition of chromatin remodeling using biophysical, biochemical, and cell-based methods.• Expressed and purified multiple constructs of CHD1L from E. coli.• Evaluated the impact of CHD1L expression in colorectal cancer patients using RNA-seq and microarray data.• Characterized the function of CHD1L on TCF-transcription using RNA silencing and gene overexpression.• Developed an CHD1L in vitro assay and carried high-throughput screen… Show more • Developed in vitro assays to characterize inhibition of chromatin remodeling using biophysical, biochemical, and cell-based methods.• Expressed and purified multiple constructs of CHD1L from E. coli.• Evaluated the impact of CHD1L expression in colorectal cancer patients using RNA-seq and microarray data.• Characterized the function of CHD1L on TCF-transcription using RNA silencing and gene overexpression.• Developed an CHD1L in vitro assay and carried high-throughput screen (20,000 compounds) using automated liquid handling (PerkinElmer Janus).• Identified small molecule inhibitors of CHD1L and characterized their activity using biochemistry, cell biology, and 3D high-content analysis using tumor organoid models (PerkinElmer Opera Phenix). Show less

Ann Arbor, Mi

• Characterized inhibitors of histone methyltransferases NSD1, NSD2, and NSD3 as well as BCL6 .• Expressed and purified multiple histone methyltransferase proteins for enzyme assays and structural biology efforts.• Utilized biophysical methods (NMR, x-ray crystallography, isothermal calorimetry, computer aided drug design) to characterize small molecule-protein interactions.• Structure-based computational drug design using Discovery studios and molecular dynamics simulations.

Ann Arbor, Mi

Working with PIH Engage to build local communities dedicated to education and advocacy for global health and health inequality issues.

Kalamazoo, Michigan Area

Worked in the Smith lab synthesizing a series of marcocyclic vanadium compounds to understand the influence on molecular structure and electrophysical properties.

In lab teaching assistant and grader for Organic Chemistry I and II.

Ann Arbor, Mi

Worked in the Paul Hollenberg's lab in the Department of Pharmacology at a part of the NSF sponsored Interdisciplinary Structure and Function of Proteins REU program. Research focused on the P450 CYP2B6 and its polymorphisms. I helped to develop an in-vitro assay to detect the metabolism of cyclophosphamide by CYP2B6 using fluorescence coupled with HPLC.

Doctor Of Philosophy (Phd), Biochemistry And Structural Biology

Bachelor Of Arts (B.A.), Chemistry, Biological Physics, Biochemistry And Molecular Biology

Activities and Societies: American Chemical Society, Student Commission

High School Diploma