Akshay Neeli Email & Phone Number

Somerville, Massachusetts, Us

- Implement and validate cGMP test methods involved in the manufacturing of bluebird bio drugs.- Scientific methods include flow cytometry, gene/protein expression, cell counting, cell culture analysis, qPCR, mass spectrometry etc.- Collaborate with CxOs around the world to oversee method executions.- Author technical documents such as SOPs, validation reports, MAAs/BLAs. - Support investigations and deviations related to drug product release and stability methods.- Consistently exposed to DoE, CAPA, CMC documentation and regulatory filings and statistical data analysis.

Lawrence Township, Nj, Us

• Solved the problem of Protein Low Molecular Weight (LMW) species formation in BMS drugs to increase purity by 90%. • Patent filed (one of three inventors).• Established the ability of cytochrome p-450 oxidase to inhibit thioredoxin and reduce LMW species formation.

Cambridge, Massachusetts, Us

• Worked on novel technology called Acoustophoretic Bioprinting. Established 100% protein stability under strong acoustic fields through morphology and binding studies. • Became first ever team to successfully develop monodisperse microscopic polymers encapsulated with 70% mAb concentration for applications in subcutaneous drug delivery.

Manipal, Karnataka, In

• Proved that DNA Methylation influences Glucose Metabolism at a larger extent during disease progression. • Proved that MDA-MB-231 breast cancer cells show a 40% increase in adaptation to glucose depletion when compared to HepG2 cells.• Established TPI1 and PYGL gene targets for improved control of glucose metabolism under the influence of DNA methylation.• Successfully defended thesis.

Hyderabad, Telangana, In

- Spent the internship rotating labs and repeatedly conducting several laboratory techniques to gain high levels of proficiency. - Some of these techniques include Mammalian and Microbial Cell Culturing, Cell Line Development, Bioreactor Operation, Transfection techniques, DNA/RNA Extraction and Analysis, Gel Electrophoresis, SDS-PAGE, PCR, Western Blot, Southern Blot, HPLC, Flow Cytometry, Bioinformatic tools, Laboratory Safety Measures, Media Preparations and Chemical Compositions.repeatedly to gain high levels proficiency.

Bangalore, Karnataka, In

- Worked on large scale waste water treatment (Reverse Osmosis).- During the process of treatment, inactivated cell mass was being produced in large amounts.- Constant land filling of the cell mass is expensive, so my research included its re-utilization.- Developed a novel method of utilizing the cell mass in construction.- Patent expected in 2020. Used techniques such as COD (Chemical Oxygen Demand) Analysis, BOD (Biochemical Oxygen Demand) Analysis, pH probes, Dissolved Oxygen probes, Carbon Dioxide Probes, Bio-sensors for pressure and temperature changes, Thiol testing using Ellman's reagent, Immunoperoxidase assays and Immunofluorescence for the detection of viruses, Gram Stain Tests, Streak and Stab Microbial cultures, Broth Cultures, Virus Cultures, CAMP Test, Endospore Staining, Giemsa Staining, Bacteriological Water Analysis, Mammalian Cell Culture Methods and MTT Cytotoxicity Assays.

Master Of Science - Ms, Biotechnology

Bachelor Of Science - Bs, Biotechnology