Barielba Nenbee M.Sc. Email & Phone Number

Plum Island

Atlanta, Georgia, United States

• Perform advanced genetic characterization of hepatotropic viruses • Develop and maintain Illumina-based, next-generation sequencing workflows for the identification of viral hepatitis transmission networks in different epidemiological settings• Development and optimization of next generation sequencing laboratory workflows for molecular characterization of entire mitochondrial DNA among Hepatitis C positive infected individuals• Amplicon deep sequencing for characterization of highly variable regions along viral genomes including hepatitis A and C• Develop and optimize next generation sequencing laboratory workflows, including DNA library preps and MiSeq sequencing runs, for HAV and HCV genetic characterization• Maintain a large production environment comprising seven highly integrated Beckman Biomek liquid handlers (NX, FX and i7) furnished for sample aliquoting, primer aliquoting, plate stamping, and DNA library prep• Validate and maintain BSL2 laboratory environment and processes• Serve on the branch quality and safety committee, developing solutions for laboratory safety

Atlanta, Georgia, United States

• Conducted interviews with patients and community members to gather information on infectious disease contacts and related health history• Located individuals reported to have tuberculosis or in contact with infected individuals, acting as a liaison with hospitals and health providers• Trained and educated patients and health providers on tuberculosis transmission and treatment• Performed tuberculin skin testing, daily directly observed therapy, and collected sputum specimens• Managed patient cases, prepared reports, and presented cases for review by public health officials• Assisted in Monkeypox outbreak response efforts by training and advising health providers on vaccine application

Atlanta, Georgia, United States

• Developed experimental protocols and research objectives for incorporating of 4-ethoxy benzoic acid into polylactic acid to inhibit Staphylococcus aureus biofilm using additive manufacturing tools • Analyzed microstructure and composition of Staphylococcus aureus utilizing light and fluorescent microscopy for biomass and biofilm quantification• Cultured Yarrowia lipolytica cells and extracted cellular lipids utilizing pyrolysis for oleic acid identification and analysis by gas chromatography-mass spectrometer (GC-MS)• Prepared clear, accurate, and concise reports and presentations to laboratory personnel and management

Atlanta, Georgia, United States

• Facilitated student and staff learning through lectures and hand-on demonstrations of microbial techniques and cultivation, quantitation, isolation, and identification of BSL-1/BSL-2 bacteria • Created a metagenomic database of urban microbial communities using environmental sampling and Oxford nanopore MinION next-generation sequencing • Successfully transitioned a course from in-person to a hybrid model by creating online content and establishing shared databases• Enforced laboratory safety and biohazard protocols to maintain a safe work environment

Atlanta, Georgia, United States

• Investigated Salmonella enterica serotype Typhimurium flagellin monomer, FliC, as a scalable recombinant vaccine platform through expression and purification • Investigated the production and purification of asparaginase enzyme from Rhodococcus rhodochrous DAP 96253 using fermentation and AKTA chromatography• Created and implemented amino acid thin layer chromatography for asparaginase and glutaminase detection• Contributed to the investigation of non-contact delayed fruit ripening and fungal growth of climatic fruit post-harvest utilizing induced Rhodococcus rhodochrous DAP 96253 volatile organic compounds

Master Of Science - Ms, Applied And Environmental Microbiology

Bachelor Of Science - Bs, Cell/Cellular And Molecular Biology

Biology, General