Britany Rufenach Email & Phone Number

Vancouver, BC, CA

Vancouver, British Columbia, Canada

Uses biosensor-based technology and biophysical assays for screening of antibodies to find desired kinetic, epitope, and developability profiles and enable rapid selection of lead drug candidate molecules.-Uses surface plasmon resonance (SPR) to determine binding kinetics, affinity, and mechanism of action of antibodies-Characterizes interactions with.

Vancouver, British Columbia, Canada

• Studied membrane protein complexes involved in muscle excitation-contraction coupling via cryo-EM, x-ray crystallography, NMR, and biophysical characterization of protein-protein interactions.
• Developed purification protocols for challenging soluble and membrane protein targets, with demonstrated expertise in use of AKTA systems and purification techniques – affinity, IEX, HIC, SEC
• Biophysically characterized diverse protein-protein interactions by ITC and NMR to elucidate affinities, thermodynamics, and binding mechanisms
• Solved high resolution structures of protein complexes via x-ray crystallography
• Used single particle cryo-EM to determine structures of membrane proteins bound to peptides and regulatory proteins
• Trained and supervised three undergraduate thesis students in completion of independent projectsSkills: Cloning

• With other graduate students, co-founded a science communication initiative to provide evidence-based information to the public about their mental health questions.Skills: Writing
• Science communication
• Editing

• Developed and optimized a high-throughput assay for screening of mutant protein receptor function
• Performed live cell fluorescence imaging and data analysis
• Gained extensive knowledge of tissue culture, transfection of mammalian cells, and generation of stable-expressing cell linesSkills: Mammalian tissue culture
• Assay development
• Microscopy

Lyon Area, France

• Studied the effects of dopamine receptor mutations and RNAi knockdown on sleep and learning pathways in Drosophila
• Performed whole brain dissections, immunostaining, and fluorescence microscopy to assay protein activity

Ottawa, Canada Area

• Contributed to the development of a qPCR-based assay to detect Legionella in HVAC water samples
• Developed a quality control procedure for reagent manufacturing; conducted extensive quality control experiments prior to product release

Doctor Of Philosophy - Phd, Biochemistry And Molecular Biology

• CIHR Doctoral Fellowship - 2022-Present • Four Year Fellowship – 2020-2022 • Josephine T Berthier Fellowship - 2019 • Canada Graduate.

Honours Bachelor Of Science, Specialization In Biochemistry

• CGPA 9.1 • Christian and Christian Detellier Schoalrship in Science 2018 • Navan Lions Club Science Scholarship 2018 • Gilles G. Patry.