Britany Rufenach Email & Phone Number

Vancouver, Bc, Ca

Vancouver, British Columbia, Canada

Uses biosensor-based technology and biophysical assays for screening of antibodies to find desired kinetic, epitope, and developability profiles and enable rapid selection of lead drug candidate molecules.-Uses surface plasmon resonance (SPR) to determine binding kinetics, affinity, and mechanism of action of antibodies-Characterizes interactions with target antigens, FcRn, and Fc gamma receptors-Supports high throughput workflows for analysis of the biophysical characteristics of hundreds of antibody molecules- Supports detailed mechanism-of-action and stability studies to select lead candidates- Manages internal and external projects, communicating complex data to partners and multidisciplinary teams- Collaborates with data scientists and other teams to streamline collection and analysis of large data sets

Vancouver, British Columbia, Canada

Studied membrane protein complexes involved in muscle excitation-contraction coupling via cryo-EM, x-ray crystallography, NMR, and biophysical characterization of protein-protein interactions.• Developed purification protocols for challenging soluble and membrane protein targets, with demonstrated expertise in use of AKTA systems and purification techniques – affinity, IEX, HIC, SEC• Biophysically characterized diverse protein-protein interactions by ITC and NMR to elucidate affinities, thermodynamics, and binding mechanisms• Solved high resolution structures of protein complexes via x-ray crystallography• Used single particle cryo-EM to determine structures of membrane proteins bound to peptides and regulatory proteins• Trained and supervised three undergraduate thesis students in completion of independent projectsSkills: Cloning • Recombinant protein expression • Protein purification • CryoEM • Negative stain • X-ray crystallography • NMR • Single channel electrophysiology • PyMol • Chimera

With other graduate students, co-founded a science communication initiative to provide evidence-based information to the public about their mental health questions.Skills: Writing • Science communication • Editing

• Developed and optimized a high-throughput assay for screening of mutant protein receptor function• Performed live cell fluorescence imaging and data analysis• Gained extensive knowledge of tissue culture, transfection of mammalian cells, and generation of stable-expressing cell linesSkills: Mammalian tissue culture • Assay development • Microscopy

Lyon Area, France

• Studied the effects of dopamine receptor mutations and RNAi knockdown on sleep and learning pathways in Drosophila • Performed whole brain dissections, immunostaining, and fluorescence microscopy to assay protein activity

Ottawa, Canada Area

• Contributed to the development of a qPCR-based assay to detect Legionella in HVAC water samples• Developed a quality control procedure for reagent manufacturing; conducted extensive quality control experiments prior to product release

Doctor Of Philosophy - Phd, Biochemistry And Molecular Biology

• CIHR Doctoral Fellowship - 2022-Present • Four Year Fellowship – 2020-2022 • Josephine T Berthier Fellowship - 2019 • Canada Graduate.

Honours Bachelor Of Science, Specialization In Biochemistry

• CGPA 9.1 • Christian and Christian Detellier Schoalrship in Science 2018 • Navan Lions Club Science Scholarship 2018 • Gilles G. Patry.