David B. Email & Phone Number

Raleigh-Durham, North Carolina Area

Raleigh-Durham, North Carolina Area

Develop new products and provide technical support to improve or extend the use of existing products, methods and techniques. Perform proteomic studies using the Sciex TripleTOF 5600 System.

Rtp, Nc

Mass Spec Bioanalytical Support o Provide biological mass spec support for RTP including targeted follow-up method development from global metabolomics.o Skilled chromatographer with broad analytical experience including nanoLC, laminar flow, and dual column systems. o Provide protein sequencing using enzymatic digestion and LC/MS/MS-based peptide sequencing using Mascot Distiller.o Advanced troubleshooting LC and MS skills.Drug Metabolismo Provide MS-based analytical support for in-vitro metabolism studies including metabolism, permeation, inhibition, TDI and special studies aimed at assessing drug discovery compounds as potential lead candidate drugs. o Develop LC/MS/MS methods using AB/Sciex 4000 triple quadrupole mass spectrometers for high throughput in vitro metabolism screens. Maintain shared LC/MS/MS method database.o Performed metabolic stability assays (S9 and microsomes). o Proficient on Tecan Genesis and Biomek FX liquid automation platforms.Information Technology o Created and implemented automation strategies for analysis for both in vivo and in-vitro research including in-house Excel macros to process and report metabolic stability, permeation, and CYP450 inhibition/TDI data.o Serve on multiple business unit team to create software tools to automate data processing and reduction. o Serve as team champion of AB/Sciex Analyst, and Excel software. Administrator of department file shares and developed database-driven AV DPU Science forum website.Othero Served as safety chair for ID MDD DMPK.

o Developed and implemented analytical methods to analyze endogenous levels of small molecules, organic acids, amino acids, and elements in tissue for PGI’s proprietary metabolomics platforms. o Ran the elemental analysis platform using ICP-MS for the high throughput analysis of important elements in tissue matrices. o Established standard operating procedure for the semi quantitative analyses of metabolites in Arabidopsis that included extraction, sample prep, instrumental analyses and quantitation. o Planned, coordinated and implemented analytical laboratory builds which included the purchase and installation, of HPLC-TOFMS, GC-TOFMS, GC-FID, and ICP-MS.

o Responsible for developing and implementing HPLC, GC/MS (ion trap and single quadrupole), capillary electrophoresis and accelerated solvent extraction analytical methods for analysis of pesticide residues in animal matrices. This entailed conducting studies upholding GLP compliance through adherence to SOP’s, and maintaining accurate records. o Accountable for maintaining and ordering laboratory and instrument supplies. Performed preventative and routine maintenance on HPLC, GC/MS, accelerated solvent extraction and capillary electrophoresis. o Drafted and finalized GLP reports for submission to the EPA to support registration of new active ingredients for method validation, accountability and related studies.

Conduct project investigating calcium bonding to a series of synthetic, peptides, using capillary electrophoresis under the supervision of Ruth Anne Henriksen.

Conducted ID testing on raw materials for use in cGMP manufacturing according to GLP standards.