Caroline Roth Email & Phone Number

Durham, North Carolina

Durham, North Carolina, United States

Morrisville, North Carolina, United States

Durham, North Carolina

Dave lab: http://davelab.org/Principal investigator: Sandeep Dave, MD, MS, MBA-Research on lymphomas trying to define cells of origin and genes involved by using CRISPR-Cas9 systems in cell culture as well as in mouse models, under the guidance of our computational team analyzing sequencing data from tumor samples. -Within the past few months, I have taken on the coordination of onboarding many collaborators at institutions around the world, as well as the coordination of sample and data collection for the lab’s numerous ongoing projects. -Cell Culture (primary & established), CRISPR-Cas9 in human and mouse cells, BSL-2 animal handling, Mouse Husbandry, Genotyping, Injection (tail vein), Bleeding (sub- mandibular, cardiac puncture), Dissection and Organ Harvest, Tissue Processing for Flow and Histology, Flow Cytometry and Sorter (Sony SH800)

Chapel Hill

HIV Cure Center at UNC - Dr. MargolisPrincipal investigator: Dr. Carolina GarridoSchool of Medicine, Department of Infectious diseases and Global HealthI am thrilled to have joined Dr. Margolis' team, and excited to be working with Dr. Garrido on NK cells. The team is currently working on developing and validating anti-HIV-1 cell therapies using NK cells as well as Dual-affinity re-targeting (DART) antibodies. We are looking to deepen our understanding of NK functions, especially during HIV infection, and of how to harness these in order to fight HIV. We are trying to develop NK cells (transduced or activated and expanded under specific conditions) to use as potential treatment for HIV. For that purpose we are developing in parallel a mouse model (in NSG) to verify our findings.Using: Work in BSL-2+, Cell Culture of established cell lines and primary cells from blood (healthy and infected subjects), immuno-magnetic cell sorting, cell transduction with retrovirus, BSL-2 animal handling, Mouse injection (IP, IV, SubCu, IM) and bleeding (tail nick, sub-mandibular, cardiac puncture), kit ELISAs, Virus and Cell Assays, Flow Cytometry (Attune), Muse cell analyzer

Parnassus Campus

The Laboratory for Tumor and AIDS Virus Research, Principal investigator: Dr. Jay A. Levy, M.D.Department of Medicine, Division of Hematology and OncologyThe team is focusing on finding, validating, and developing anti-HIV-1, and, more broadly anti-viral proteins. We are looking into the CD8+ cell non-cytotoxic control of HIV-1, and the precise pathways activated in this condition. Dr. Levy and I successfully wrote and submitted an R21 grant with the NIH, which we were awarded. We are also involved in a stem cell project, in which the team is developing a way to mutate patients iPS cells to make them resistant to HIV-1 infection. The goal being to safely re-introduce the mutated iPS cells back into patient for treatment and possibly a cure. Mice study with human iPS cells are on going.Using: Cell Culture of established cell lines and primary cells from blood, immuno-magnetic cell sorting, cell differentiation, cell transfection and electroporation, bacterial transfection, MaxiPreps, kit ELISAs and in house ELISAs, Virus Assay, Radioactive and Colorimetric RT Assay, Flow Cytometry (LSRII, LEGENDplex, FlowJo), SDS-PAGE, Western Blot, Native Gels, Protein and Nucleic acid extraction, RT-PCR, q-PCR, PCR, sequencing, bioinformatics, protein immunoprecipitation.

Being organized, reliable, with great communication skills and a good team player, as well as providing excellent customer service are all important characters for this job. I truly enjoy being in contact with our food production system through an organic producing farm, and being able to influence and educate customer on their decision to buy and eat specific food items. I truly believe that health starts with the food we all ingest and by working with and promoting what I believe is the very beginning of healthy habits I feel very rewarded, just the same way I am with working in the lab and being able to find ways of improving everyone’s life through research and discoveries.

Hayward, Ca

-Research on cloning GBV-C’s RNA-dependent RNA polymerase, NS5B-Bioinformatics, molecular cloning, PCR, primer design, bacterial transformation, sequencing, protein expression, protein purification, SDS-PAGE

Hayward, Ca

-Taught Introduction to Biology Laboratory and Cellular and Molecular Biology Labs for undergraduate students. Designed and assessed student work, including quizzes, mid-terms, finals and lab reports. Planned lab schedules for each quarter and trained new teaching associates.-Participated in re-designing some classes (lessons and experiments) in the quarter to semester change at CSUEB.

Purpan Toulouse, France

-Investigated HIV-1 latency and activation by testing different promoter activation signals such as de-methylation treatment, inhibition of de-acetylation, and co-infection with Cytomegalovirus -Mammalian cell culture, virus culture and titration, SDS-PAGE, ELISA, Western Blots, FACS, worked in a Biosafety Level 3 Laboratory

Master'S Degree, Biological Sciences - Cellular And Molecular Biology, 3.9

English And Gre

English

Master'S Degree, Immunology And Infectious Diseases

Bachelor'S Degree, Biochemistry And Molecular Biology

Associate Of Science (A.S.), Life Sciences