Primary Technologist to set-up and run all aspects of emerging Molecular Lab.Responsible for creating and implement some QC, workflow, editing SOPs, performing validations, implementation of new methodologies and reporting.

EDR stands for Extreme Drug Resistance Assay. It helps determine the probability of a tumor’s sensitivity to specific chemotherapy drugs. The test is done on a patient’s tumor rather then the patient. The test is done on the cultured tumor rather then the patient in order to reduce additional toxic exposure or uneffective chemotherepy drugs to the patient. Then a report is generated with probability of resistance for each chemotherapy drug.

Direct sequencing-based assays using ABI Prism 3730 Gene Sequencers Reporting out clinical results to clients.Overseas and monitors Turn Around Time, Reagents, QC and maintenance of reagents and robots for several benches.Responsible for the supervision of non-licensed staff members.PCR, qPCR, using, Biomek robot, ABI Prism 7900HT, and manual set-ups.

General Molecular Biology techniques to identify mutations of specific genes. Some work includes DNA extraction both automated and manual, various PCRs (OLA, multiplexes, and Real-Time PCRs), and various forms of detection (Sequencing, chemiluminecense, gel electrophoresis, and Southern Blots).

Perform large volume clinical molecular genetic testing.Sample intake, processing, large volume automated DNA extraction on multiple platforms, manual extractions, and buccal swab extraction.Gel Electrophoresis, Northern, Southern, and Western Blots.Genetic mutation detection using chemiluminecense.Molecular Genetics workflow committee member

Karyotyping metaphase images of various tissue types (blood, bone marrow, chorionic villi, amniocyte fibroblast, and lymphomas). Utilizing digital imaging system software (PSI) to capture metaphase images Some microscopy, and maintaining department case archive.Blood, Bone Marrow, Amniocyte, and Tissue set-up, culture, and harvest. Basic FISH (fluorescence in situ hybridization) technique for molecular hybridization for specific genetic mutations and slide preparation and staining. ADDITIONAL PROFESSIONAL SKILLSWide general operating knowledge of Microsoft Office, Outlook Express, Windows, Word, Excel, PowerPoint, Internet Explorer, PC, and Macintosh platforms.Proficient in multiple aliquoiting robots: Qiagen, Tecan, and Biomek; Trained in using ABI 3100, 3130, 3730 sequencers, Genemapper and Seqscape software. Trained in using ABI 7900HT Real Time lightcycler, Roche 480 lightcycler, and various thermal cyclers.Reagent preparation; Organize and prepare clinical specimens for testing. Prepare testing equipment, reagents, and controls for the laboratory.Preventative maintenance/trouble-shooting of various robots, machines, and equipment and performs calibrations. Trained in sterile technique; knowledge of GLPs, QA/QC, CAP, CLIA, New York State and California, and ISO regulations, Six Sigma trained; proper use of PPE; handling of specimens, hazardous materials, and biological waste.Aseptic Technique/Clean Room Training and SOP author and revision