David Waugh Email & Phone Number

Nutley, Nj

I participated in a structure-based drug design team by leading a group focusing on the overproduction and purification of recombinant proteins for X-ray and NMR studies. I also developed new technologies for protein expression & affinity purification, including the first vector for expression of biotin-tagged proteins in E. coli (Tsao et al.,Gene, 1996, 59-64). I also pioneered the development of E. coli BL21(DE3) mutants with genetic lesions created by phage transducion that were designed to prevent cross-labeling of other amino acids, for NMR experiments where it was desirable to label only one or a few amino acids with !5-N (Waugh, D.S., J. Biomol. NMR, 1996, 184-192), I have prepared dozens of stable isotopically labeled proteins, including 15N, 13C and 2H, for NMR studies. Both of these endeavors have resulted in a multitude of requests for biological materials. I have deposited the Biotin acceptor peptide vector with Addgene, so I don't get too many personal requests for that anymore, although Addgene does. Unfortunately, I have yet to find a repository for the he genetically modified E. coli strains I created. They are still available only from me.

Cambridge, Ma

I was a postdoctoral fellow in Dr. Robert Sauer's laboratory in the Department of Biology. I was funded by an NIH individual postdoc award. I conceived of and worked independently on a new project that I brought to the lab myself, which involved structure-function relationships in the type IIS restriction endonuclease FokI.

Under the direction of Dr. Norman Pace, I carried out an extensive mutational analysis of the RNA enzyme ribonuclease P, seeking to identify elements that are critical for its catalytic function. I received a teaching award in my first year of graduate school and was the recipient of the Esther L. Kinsley award for best Ph.D. dissertation presented to the Graduate School at Indiana University in 1989 (all departments).

Cambridge, Ma

I worked in the laboratory of Prof. Nancy Kleckner, in the Department of Biochemistry and Biophysics, where I studied the regulation of transposition by the Tn10 transposon in Escherichia coli.

New Haven, Ct

I worked in the laboratory of Prof. Terry Platt, in the Department of Molecular Biophysics & Biochemistry, where I studied the mechanism of transcription termination in Escherichia coli, and later in the laboratory of Prof. Dieter Soll, where I studied the specificity of novel suppressor tRNAs in E. coli.

Biology

postdoc in Bob Sauer's lab

Ph.D., Biochemistry & Molecular Biology

B.S., Molecular Biophysics & Biochemistry

High-School Diploma, English Language, Creative Writing, Poetry, And Science

Activities and Societies: Music Award, 1977. Faculty Nomination Award, 2001. Greater Hartford Youth Chorale. Lead singer and keyboard.