Deepti Bhat Email & Phone Number

San Diego, California, United States

Drug discovery and development of small molecule Wnt pathway modulators, for oncology, regenerative medicine and degenerative diseases

- Provided weekly SAR support to Therapeutic Area team in finding inhibitors for their target including initial screenings (biochemical luminescent assay in 1536 format), identifying potential inhibitors using high content imaging and Opera, and completing data analysis using Harmony, 3DX, and Genedata.- Supported SAR assays for nuclear receptors and.

Greater San Diego Area

- Performed assays on CNS targets, generated target specific stable cell lines and studied MOA with assays using MSD biomarkers (chemokines or cytokines), Westerns, and ELISA assays. - Studied modulators of GPCRs (targets for metabolic diseases and inflammation). - Cloned inducible constructs and generated stable single clones to study expression of gene.

San Diego, California, United States

- Performed drug target discovery biology (cell and molecular) assays for metabolic diseases: novel target validation for diabetes and obesity, generate target specific cell lines, gene knockdown and over expression, cell and tissue RNA expression by qRT-PCR, cellular HTS assay development for high throughput screening, and SAR support performing.

Greater San Diego Area

- Elucidated the effect of small molecule compounds on the expression of B-Raf, C-Raf genes and the pathways involved. - Understanding of the newly identified pathways using siRNA mediated knock down of specific genes of interest. - Assays used were Western blots, siRNA method to knockdown selected genes in the pathway, understand MOA in different cell.

Greater San Diego Area

- Identified and characterized promoters using high throughput fluorescent and luminescent assays with the objective of modifying seed oil content to increase yield. - Assays included PCR, cloning, Northern blots and Southern blot analysis to understand expression of the transgene

Greater St. Louis Area

- Identified and characterized tissue specific promoters in Arabidopsis and soybean plant. - Cellular expression and localization of the gene using immunohistochemistry and/or in situ hybridization. - Assays included PCR, cloning promoters in vectors or in T-Rex system, measure promoter expression using Gus as the reporter gene, Northern blots and Southern.

Master Of Science - Ms, Biochemistry

Bachelor Of Science - Bs, Biochemistry