Deepti Bhat Email & Phone Number

San Diego, California, United States

Drug discovery and development of small molecule Wnt pathway modulators, for oncology, regenerative medicine and degenerative diseases

- Provided weekly SAR support to Therapeutic Area team in finding inhibitors for their target including initial screenings (biochemical luminescent assay in 1536 format), identifying potential inhibitors using high content imaging and Opera, and completing data analysis using Harmony, 3DX, and Genedata.- Supported SAR assays for nuclear receptors and performed transient transfection assay - As part of all drug discovery project flow schemes, ran [3H] dofetilide-binding assay as a screening tool for hERG assays

Greater San Diego Area

- Performed assays on CNS targets, generated target specific stable cell lines and studied MOA with assays using MSD biomarkers (chemokines or cytokines), Westerns, and ELISA assays. - Studied modulators of GPCRs (targets for metabolic diseases and inflammation). - Cloned inducible constructs and generated stable single clones to study expression of gene.

San Diego, California, United States

- Performed drug target discovery biology (cell and molecular) assays for metabolic diseases: novel target validation for diabetes and obesity, generate target specific cell lines, gene knockdown and over expression, cell and tissue RNA expression by qRT-PCR, cellular HTS assay development for high throughput screening, and SAR support performing pharmacology and mechanism of action studies. - Cystic Fibrosis Research: Developed cellular assays to identify small molecule correctors of CFTR protein to stabilize the NBD1-MSD2 interface. Using cell lines expressing CFTR and mutant variants, developed cellular HTS and functional assays. Worked with medicinal chemistry team to identify small molecule correctors to improve CFTR function. Screened for and identified small and large molecules that up regulate ApoA1 in cells.- Insulin Sensitizer project: Screened for and identified small and large molecule compounds that modify insulin response. Generated insulin resistant muscle cell model cell lines using FFA and/or excess amino acids; identified small and biologics molecules that could modify or interfere with various metabolic disease pathways.

Greater San Diego Area

- Elucidated the effect of small molecule compounds on the expression of B-Raf, C-Raf genes and the pathways involved. - Understanding of the newly identified pathways using siRNA mediated knock down of specific genes of interest. - Assays used were Western blots, siRNA method to knockdown selected genes in the pathway, understand MOA in different cell lines, Western blots and qRT-PCR to confirm the expression of protein or genes.

Greater San Diego Area

- Identified and characterized promoters using high throughput fluorescent and luminescent assays with the objective of modifying seed oil content to increase yield. - Assays included PCR, cloning, Northern blots and Southern blot analysis to understand expression of the transgene

Greater St. Louis Area

- Identified and characterized tissue specific promoters in Arabidopsis and soybean plant. - Cellular expression and localization of the gene using immunohistochemistry and/or in situ hybridization. - Assays included PCR, cloning promoters in vectors or in T-Rex system, measure promoter expression using Gus as the reporter gene, Northern blots and Southern blot analysis to understand expression of the endogenous gene, characterizing expression of genes at their cellular level.

Master Of Science - Ms, Biochemistry

Bachelor Of Science - Bs, Biochemistry