Denis Mccarthy Email & Phone Number

Supervising the upstream and media manufacturing process.

Senior Upstream production specialist in a new start up biopharmaceutical site.

Carlow

Supervising the vial and syringe formulations shifts

Carlow

Cork

• Performed extensive work across both Upstream & Downstream processes.
• SME for Bioreactors, Harvest and Media/Buffer processes. Trained in Cell Culture (Media Make-Up, Inoculation, Bioreactor, Centrifuge, Harvest) and Purification (Buffers, Chromatography, Viral Filtration, Bulk Fill).
• SME in site inventory control system (MAPS AS400).
• Involved in coordination and performance of dispensing during site start up.
• Performed first line maintenance & calibrated process equipment (pH, DO and pCO2 probes, as well as pH/conductivity meters, pumps, filtration Systems).
• Performed extensive CIP & SIP of vessels and equipment.

Sligo

• Performed bulk immuno-bioassays on the components of the viral diagnostic kits produced on the site.
• Performed extensive maintenance on axsym and architect immuno-assayers.
• Ensured quality of diagnostic kits matched required standards.
• Carried out the large variety of assays involved at all times to GMP standards strictly adhering to batch records involved.
• Operated stock system (BPCS AS400) and generated stock reports for when kits were passed fit for quality.
• Performed aseptic techniques during mixing of components of some diagnostic kits prior to running assays.Key Achievement: One of an elite number who were fully trained on testing of all of the large variety of.

Limerick

• Received data of interest from scientific publications for our client, a major pharmaceutical corporation.
• Entered data involved into a database based on keywords and on whether comments were negative or positive. Key Achievement: Rewarded for achieving highest level of productivity output amongst the team dealing with our.

Cork

• Cloned genes of interest in the field of apoptosis into a number of different vectors, using various techniques, such as TOPO and restriction site cloning.
• Designed PCR primers used to amplify these genes using computer software.
• Amplified up genes of interest from cDNA pool, using PCR from gene arrays developed onsite.
• Created this cDNA pool from different animal RNA sources by reverse compliment PCR.
• Obtained positive clones using RFLP.
• Prepped positive clones for sequence analysis. Genes which were found to have good sequences were then transfected into cells for analysis.

Msc, Biotechnology

Bsc, Biochemistry