Edgar Hernandez Email & Phone Number

New York, New York, United States

Perform molecular biology and biochemical procedures in development of gene therapy strategies for preclinical studies.+ Screen effects of in-house engineered gene elements on resultant expression of therapeutic targets in different cell models.+ Manage team members in meeting timelines for advancing project needs.+ Manufacture adeno-associated vectors for in-vitro and in-vivo preclinical screening and conduct in-house quality control (QC) testing.+ Perform capsid engineering of adeno-associated vectors for improved tissue tropism and transduction efficiency.Key Skills:Adeno-associated Vector Production; AKTA; Affinity Chromatography; Anion Exchange Chromatography; Process Development; Flow Cytometry; Western Blotting; Molecular Cloning; Transfection; DNA and RNA Extraction; qRT-PCR; qPCR; Immunofluorescence; CRISPR; Stable Cell Line Generation; Fluorescence Microscopy; Confocal Microscopy; Cell Imaging

New York, New York, United States

New York, New York, United States

New York, New York, United States

Performed molecular biology and biochemical procedures in elucidation and exploitation of the Ubiquitin-26s proteasome system for therapeutic purposes. Tested PROTACs for targeted ubiquitination of target proteins for pharmacological purposes. Generated and validated doxycycline-inducible and CRISPR-modified stable cells lines.Key Accomplishments:+ Led experiments for a collaboration between an early stage start-up and the Pagano lab focused on developing a novel new cancer treatment.+ Implemented a project / experiment planning procedure – including a precise visual project outline – that speeds up project completion by minimizing disruptions during experiments.+ Developed an outline for a project to identify whether an experimental compound could bind two proteins of choice – an outline that effectively validated the experiment and provided foresight into potential results.Key Skills:Western Blotting; Molecular Cloning; Transfection; Immunoprecipitation; PCR; Immunofluorescence; CRISPR; Fluorescence Microscopy; Confocal Microscopy; Cell Imaging

New York, New York, United States

Performed molecular biology and biochemical procedures in development of gene therapy strategies for clinical studies. Manufactured adenoviral and adeno-associated clinical vectors for clinical trials and conducted in-house quality control (QC) testing. Maintained cell lines and distributed reagents.Key Accomplishments:+ Validated and developed SOPs for a concept to partially automate virus purification, a process optimization that cut purification time in half and increased overall yields.+ Partnered in modernizing the in-house QC program by replacing a legacy plaque assay-based procedure with a new qPCR-based assay procedure that reduced testing times by several weeks. Served as a SME on the new procedure.+ Developed clear project plans and associated report templates to streamline project delivery. Recognized for consistently completing QC assays and submitting results several weeks ahead of deadlines.Key Skills:Adenoviral and Adeno-associated Vector Production; BSL-3; Process Development; Real-Time PCR; TaqMan; Microscopy; Cell Culture; Western Blotting; ELISA; SDS-PAGE

New York, New York, United States

Managed large quantities of research data for the global producer of flavors, fragrances, and cosmetic actives. Input and updated fragrance sample data into an inventory database for subsequent entry into an inventory management machine. Trained new hires on the data management system and associated processes.Key Accomplishments:+ Earned praise for learning BatchMetrics, the company’s recently implemented inventory management system in under a week. Selected to train new hires on data management processes and the complex new system. + Optimized the data input process by categorizing materials prior to processing, resulting in the ability to enter ~25% more inventory into the system without compromising data integrity.Key Skills:Inventory Management Systems; Process Optimization; Data Collection; Data Management

New Rochelle, New York, United States

Spearheaded experiment design, execution, and analysis for a project to synthesize and purify a neuroprotective compound for clinical study as a potential treatment for neurodegenerative diseases. Consulted with research collaborators on potential research protocols and experimental methods.Key Skills:Vacuum Filtration; Compound Purification; Compound Synthesis; Distillation; Thin-Layer Chromatography

Master Of Science (M.S.), Pharmacology

Bachelor Of Science (B.S.), Biochemistry