Eric Traub Email & Phone Number

Saline, Mi, Us

Ann Arbor, Michigan, United States

We make a super absorbent biopolymer though performing a fermentation process with a gram-positive bacteria. I can carry out every part of the upstream process from cell banking to fermentations on a 10-100L scale; which includes performing the analytical and QC (HPLC and various other assays applicable to our fermentation) and carrying out R&D experiments in order to troubleshoot issues. Utilizing my past experience at Corteva, I played a pivotal role in implementing our shake flask model and… Show more We make a super absorbent biopolymer though performing a fermentation process with a gram-positive bacteria. I can carry out every part of the upstream process from cell banking to fermentations on a 10-100L scale; which includes performing the analytical and QC (HPLC and various other assays applicable to our fermentation) and carrying out R&D experiments in order to troubleshoot issues. Utilizing my past experience at Corteva, I played a pivotal role in implementing our shake flask model and thereafter using it to troubleshoot problems that have arisen on multiple occasions, including, but not limited to; reproducibility of cell banking, media-related detrimental impacts on production and seed train development for scale-up. Given the small team atmosphere with many problems to solve, our upstream team has had to effectively work across functions whether it be with the downstream processing, business applications or financial operations teams, in order to succeed in scaling up and obtaining funding. Show less

Indianapolis, Indiana Area

Systems Biology-RNA, DNA (column and High Molecular Weight), and metabolomics extractions on various Actinomycetes for characterization, sequencing and PCR analysis of strains. Made improvements in throughput and quality of RNA and DNA extraction processes. -Developed improved processing of fermentation samples for obtaining cleaner cell pellets from complex media, on samples 24well plate up to the 30L scale, which led to higher quality metabolomics and RNA extractions.-Proficient… Show more Systems Biology-RNA, DNA (column and High Molecular Weight), and metabolomics extractions on various Actinomycetes for characterization, sequencing and PCR analysis of strains. Made improvements in throughput and quality of RNA and DNA extraction processes. -Developed improved processing of fermentation samples for obtaining cleaner cell pellets from complex media, on samples 24well plate up to the 30L scale, which led to higher quality metabolomics and RNA extractions.-Proficient at microscopy, making morphology based observations, and live/dead staining. -ddPCR for identification and quantification of target sequence-Pulse Field Gel Electrophoresis for verification of High Molecular Weight DNA quality and plasmid isolation-Proficient with QiaCube, Pipettemax, ThermocyclersHigh Throughput screening of microbial strains for the production of natural compounds.-Perform strain selection on mutagenized and engineered strains using high throughput picking and liquid handling equipment-Mutagenesis of microbial strains using chemical methods-Agar selection studies using amino acid analogs and other various compounds for selection-HTP supplementation study to assist in identification of bottlenecks-Use sterile technique in handling of Streptomyces and Saccharopolyspora and other Actino species-Assist in developing workflows and DOE's to improve strain selection process through media, incubation conditions, and other various changes-Safety Focal Point for Bioengineering and Bioprocessing department-Cook Media in 20L tank using sterilization in place technique-Shake flask studies with Actinobacteria-Autoclave and dispense media using high throughput equipmentArea Safety Focal PointThis was a 2 year role that took 30% of my time. The department of 60+ people had 1-2 individuals that were ASFPs. I was responsible for running safety meetings, monthly inspections, and collaborating with EH&S to make sure the department was following all safety guidelines. Show less

Midland, Mi

As a lab technologist at Dow Chemical I have contributed to four groups; High-Throughput (HTR) Liquid Formulations, Mechanical Dispersion and Dow Coating Materials.•Name on patent that developed an HTR instrument and method for testing scrub resistance which involved extensive proof of concept work•Assisted in developing workflows and DOE’s for HTR projects by using SerialMate, Tecan, Hamilton, and UV-vis HTR instruments•Received award for success of an HTR project developing… Show more As a lab technologist at Dow Chemical I have contributed to four groups; High-Throughput (HTR) Liquid Formulations, Mechanical Dispersion and Dow Coating Materials.•Name on patent that developed an HTR instrument and method for testing scrub resistance which involved extensive proof of concept work•Assisted in developing workflows and DOE’s for HTR projects by using SerialMate, Tecan, Hamilton, and UV-vis HTR instruments•Received award for success of an HTR project developing anticorrosion package for ship stern lubricants •Assisted in the construction of two 20L reactors and three rotor-stator dispersion lines •Created standard operating procedures (SOP’s), batch records and safety documents such as compatibility charts, PPE grids, and waste characterizations•Performed epoxy and polyol reactions in a 20L reactor using technology transferred from bench top chemistry •Assisted in retrofitting an old cGMP lab for a new process•Successfully transferred dispersion technology from bench top to 100 gallon scale for alkyd, epoxy and polyurethane resins •Performed quality control on dispersions by analyzing pH, viscosity, particle size, and percent solids •Contributor to 15 CRI's, Dow's internal version of a research paper•Became group focal point for chemical inventory and shipping Show less