Eric Olsen Email & Phone Number

Bothell, Wa, Us

San Diego, Ca, Us

-Responsible for advancement of early stage programs towards pre-clinical formats -Assay development from industry and literature -Protein engineering through molecular cloning -Testing of animal samples for PK/PD

San Diego, California, United States

-Responsible for preparation of high quality leads for mouse/cyno studies including: Transient transfection of key leads using good aseptic technique Maintain various cell lines Purification by affinity chromatography of leads Optimization of proteins by preparative SEC, adaptive buffer formulation and molecular cloning Characterization of protein by LAL assay, HPLC, Flow Cytometry, Forte, DSF, -Manage timelines necessary for prompt delivery of therapeutic leads on schedule for studies -Rapid data analysis and communication with research team -Discovery of new leads for library preparation including: Immunization and isolation of key sequences from test bleeds Use a display system and a cell sorter to identify clones Characterize clones using Cell Sorter, and Flow Cytometer Acted as head of the team during manager's maternity leave

- Preform all duties necessary to purify proteins for the research team including: Purify proteins by affinity chromatography, Assist in antibody development Formulation development Experienced in scaling up of protein expression Characterize proteins by SDS-page, ELISA, Forte, LAL, Analytical HPLC, Preparative SEC, Flow Cytometry -Preform all duties necessary to express proteins including: Transient transfection of various cell lines Cell banking Maintaining multiple cell lines for entire research team-Engage in molecular cloning and verification by DNA sequencing -Maintain and adapt Standard Operating Protocols (SOPs), batch records, etc. -Proficiency in instrument software, data analysis and data presentation -Engage in rapid communication with development team-Ability to thrive under pressure with minimal supervision

Assistant Supervisor for team of 12 associates, purifying ~70g of antibody monthly. Proficient in aseptic technique, and utilizing a FPLC daily. Wrote and edited SOPs for manufacturing use, as well as co-opted new techniques from primary literature.

San Diego

Preform all duties to purify antibodies from hybridoma and ascites. Proficient in all chromatography methods including affinity, ion exchange, size exclusion and more. Work in a team of 10 associates purifying ~30g monthly. Prepare customer orders. Keep good batch records in accordance with ISO regulations.

San Diego

Harvest cell culture, categorize and freeze raw cell material. Assist in research by maintaining stock of sterile glassware and supplies Prepare customer orders

Masters, Biochemistry

Bachelor'S Degree, Biology, General

Emphasis on molecular biology and research methods. Minor in Chemistry