Fen Chen Email & Phone Number

United States

75 Sidney Street, Cambridge, Ma02139

1. Screened different AAV capsids for HD project in neuroblastoma cell line (SH-SY5Y) , astrocyte cell line (U251) and primary astrocytes isolated from dog spinal cord and brain2. Downstream brain tissue analyses for both mRNA and protein levels to support in vivo pharmaceutical and efficacy studies in transgenic mice (YAC128 and BACHD) and lead selection and dose optimization studies in monkey3. Performed HTT, SOD1 and Frataxia cell based potency assays to support production department

Framingham, Ma

• Established a co-culture model to create a pathological condition and further screened candidate compounds targeting pathways contributing to OA• Focused on mechanism of hyalunoic acid interaction with chondrocytes and articular cartilage grafts• Set up the cell based assay in W-20-17 to screen compounds for early drug discovery of spinal fusion program• Established an apoptosis assay in DU-145 and PC-3 to screen p75 inhibitors• Understood the characterization of cell differentiations into neurons and inflammatory cells in order to create an in-vitro pain model to screen pain inhibitors• Evaluated the effect of TrkA inhibitor in blocking the activity of the activated TrkA receptor trafficking by NGF in IMR-32• Determined the effects of pan-Trk inhibition on articular cartilage and synovium in an in vitro model of OA• Established a neutral red assay for drug toxicity study

Boston, Ma

• Novel approaches to anterior cruciate ligament(ACL) healing in dogsFocused on optimization of the collagen hydrogel in vitro, further collaborated with Vanderbilt Medical Center in Nashville to create ACL defects and then repaired the defects in dogs• Gene therapeutical approach to enhance ACL healingCollaborated with Center for Molecular Orthopaedics, Brigham and Women's Hospital• Delivery of collagen hydrogel by a customer designed device attached to an arthroscope Collaborated with Dept of Mechanical Engineering, MIT• The effect of fractionation of blood on ACL fibroblasts migration, proliferation and collagen production

Cambridge, Ma

• Tissue engineered cartilage in vitro and in vivo using bovine chondrocytes with hyaluronic scaffoldsFocused on the cartilage integration using rotating bioreactors in vitro, further transplanted engineered cartilage with native cartilage into mice subcutaneously• Tissue engineered cardiac tissue using embryonic stem cell derived cardiomyocyte with hyaluronic scaffoldsFocused on characterization of embryonic stem cell derived cardiomyocytes in mice• Hybrid cardiac grafts in vitro and in vivoInvolved surgical portion, e.g. transplantation of hybrid cardiac grafts into rats subcutaneously; preparation of a strip tissue from rat hearts for tensile tests

Singapore

• Transferred of cultured chondrocytes into half of the proximal medial tibial growth plate to correct the tibiofemoral angular deformity and discrepancy of tibial length• Performed primary mesenchymal stem cell isolation and transferred them into partial growth arrest in immature rabbits • Performed cultured chondrocytes and mesenchymal stem cells in the treatment of full-thickness defects of articular cartilage in mature rabbits• Performed the effect of surgery on the iliac apophysis: an experimental study• Performed a study of morphology of chondrocytes derived from articular, iliac and costal cartilage• Performed mesenchymal cells isolation from fat to fully repair articular chondral defects• Helped Dr. E H Lee isolate mesenchymal stem cells from tibial periosteum in the treatment of partial growth arrest in children