Isaac H. Email & Phone Number

Greater San Diego Area

We make the products that I wish were available during my decade racing as a professional cyclist. Orucase is a cycling company that is revolutionizing the way people explore the world by bike.

Eastern United States

Inspired by Nature, Moved by Science. What started as a way of sustainably recycling byproducts, gave birth to the purest and fastest known beta-glucuronidase.Kura Biotec is a biotech company with offices in California and a unique enzyme extraction laboratory in Patagonia, Chile. This is how Kura Biotec manufactures, serves and guarantees the best beta-glucuronidase enzymes for analytical toxicology and diagnostic laboratories.We put consistency high on the list and have a total-control strategy “from the source to your lab”, that’s why we have been selected by many of the largest laboratories in the world, as their trusted supplier of beta-glucuronidase enzymes."Talk is Meaningless. Science Demands Data.”

Greater San Diego Area

2014-2016 - Team Champion System Stan's NoTubes 2013-2014 - Team SmartStop 2011-2013 - Team Kenda 5-Hour Energy2009-2011 - Team Mountain Khakis- Served as team captain and road manager for two years- Organized logistics, travel, allocation of resources, and strategy for 13 athletes competing in a multinational race calendar- Established relationships with prospective and existing sponsors and contributed to contract negotiations for a quarter million dollar annual budget - Ranked annually inside the top 1% of American criterium cyclists for the last six years, winning the USA Criterium series in 2010 and named top US Sprinter by Bicycle Magazine in 2013- Acted as team liaison for interviews to best represent national and global corporate sponsors

Burlington, Vermont Area

Department of Transplant Surgery: - Performed laboratory techniques including; bacterial cloning, recombinant protein production, and characterization, tissue harvesting and generation of monoclonal/polyclonal cell lines in both human and murine species- Analyzed experimental data via; intracellular staining and other immunoassaying techniques Western blot, SDS-PAGE, Flow cytometry, chromatography, ELISA- Contributed to several projects investigating NKT cell function in human and murine speciesPublications:Rymarchyk SL, Lowenstein H, Mayette J, et al. Widespread natural variation in murine natural killer T-cell number and function. Immunology. 2008;125(3):331-343. doi:10.1111/j.1365-2567.2008.02846.x.Nagaleekar VK, Sabio G, Aktan I, et al. Translational control of NKT cell cytokine production by p38 MAP kinase. Journal of immunology (Baltimore, Md : 1950). 2011;186(7):4140-4146. doi:10.4049/jimmunol.1002614.Laboratory Skills and Experience: Animal Techniques – Murine- Experienced in animal handling, intraperitoneal injections, blood collection (cardiac puncture and tail vein), euthanasia, and organ harvestingMolecular Biology - - PCR and primer design, agarose gel electrophoresis, Southern blot and production of DNAprobes, site-directed mutagenesis, general cloning techniques including the production of competent cells, TOPO cloning, plasmid DNA preps, and bacterial transformation (chemical and electroporation). Also experienced in post-transcriptional gene silencing via siRNAImmunology - - Flow Cytometry, Immunostaining of Cells, Intracellular, Surface, Cellular IrradiationELISA, Cell enrichment using Miltenyi magnetic beadsBiochemical techniques - - Chromatography, SDS-PAGE, Western Blot, Protein Expression/Purification/Modification