Isabel Masteika Email & Phone Number

Chicago, Illinois, United States

• Provide pre- and post-sales support to customers throughout the Midwest and across three instrument product families
• Train customers on instrument usage, assay development, and data analysis
• Collaborate with sales team on instrument demos and pre-sales technical presentations

Chicago, Illinois, United States

• Applied novel CRISPR-based gene activation platform to differentiate stem cells into two distinct developmental lineages; characterized differentiation using flow cytometry and functional assays
• Developed high throughput automated immunofluorescence imaging workflows
• Validated in vivo disease model for evaluating therapeutic potential of iPSC-derived cell type
• Analyzed data and presented regular updates at company meetings

Cambridge, Massachusetts, United States

• Developed high throughput luciferase reporter and toxicity screening assays
• Isolated mouse primary immune cells from tissue and analyzed cells by flow cytometry
• Optimized qPCR readouts for tissue and cell lysates across multiple projects
• Collaborated with cross-functional project teams on a total of 7 projects, meeting regular deadlines and presenting data internally and to external partners

• Implemented cell culture-based systems to model the GI barrier including transwell and gut-on-chip models
• Optimized high throughput ELISA, AlphaLISA and TR-FRET workflows for protein quantitation

• Independently designed, planned, and executed in vitro experiments modeling neuromuscular disease pathology
• Used fluorescence microscopy and semi-automated image analysis techniques to measure protein expression at the cellular level
• Optimized gene expression workflows, toxicity screens, and western blotting
• Regularly presented updates at weekly lab meetings
• Oversaw lab operations and ordering, in addition to ensuring EHS compliance and regular equipment maintenance
• Research culminated in a first author publication