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James Kearns is a Early-Stage Immunology Automation Lead – Vaccines at Sanofi. He possess expertise in microsoft office, biotechnology, histology, microsoft excel, research and 13 more skills.
Sanofi
View- Website:
- sanofi.com
- Employees:
- 88327
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Early-Stage Immunology Automation Lead – VaccinesSanofiWaltham, Ma, Us
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Senior Staff ScientistIncyte Jun 2021 - Aug 2024Wilmington, Delaware, United States• Specializing in in vitro and ex vivo assay development, high complexity, multi-color flow cytometry (panel design, development for multiple tissue types), and spatial biology to increase disease understanding. • Evaluate novel small molecule and protein therapeutic agents for dermatological immune disorders utilizing in vivo models: perform animal dosing (p.o. and i.p.), blood and tissue collection, sample processing and assay to test novel small molecule and protein therapeutic agents for dermatological immune disorders. • Support academic scientific collaboration to enhance dermatological disease understanding. • Present data and findings as well as write-up reports for regulatory and patent submissions. -
Senior Staff ScientistIncyte Jun 2020 - Jun 2021Wilmington, Delaware, United StatesI am a contract employee via Proclinical Staffing. Work included immunological in vitro and ex vivo assay development for preclinical model evaluation. Responsible for conducting animal dosing, tissue collection and sample processing and assay to test novel therapeutic agents for dermatological immune disorders.
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Scientist, B Cell ImmunologyYoh, A Day & Zimmermann Company @ Sanofi Pasteur Jul 2018 - Jun 2020Boston Ma• Multicolor flow cytometry, cell sorting, and single cell genomics aimed at B cell repertoire profiling and antibody discovery• Responsible for implementing new protocols including ELISA’s for antibody characterization, primary and secondary cell culture, and cytokine analysis using MSD and Luminex assays
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Graduate InternPro Unlimited @ Sanofi Pasteur Jan 2018 - Jun 2018Greater Boston AreaBacterial Immunology GroupConducting in vivo and in vitro experiments in the area of immunogenicity, safety and efficacy evaluation of vaccine candidates and monoclonal antibodies (mAbs) targeting various bacterial pathogens. Laboratory techniques such as ELISA, ELISPOT, SDS-PAGE, Western blot, FACS, mammalian and bacterial cell culture, light phase and fluorescent microscopy. Work in handling BSL2 infectious agents as well as small mammals for laboratory purposes. • ELISA, ELISPOT, SDS-PAGE, Western blot, FACS, microscopy and/or bacterial culture, cell-based assays• Perform sample processing from small laboratory animals, ELISA and cell-based assays in support of preclinical programs• Aseptic technique and HSE regulations handling BSL2 agents• As required perform laboratory support activities such as maintaining equipment and ordering lab supplies
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Research AssistantTufts Medical Center Aug 2015 - Nov 2018Greater Boston Area• Performed research on molecular biomarkers in prostate cancer, cancer epigenetics, and development of innovative techniques for tissue biomarker research under the direction of Dr. Sandra Gaston.• Gene expression data analysis and presentation and was completed foremost with Excel and Graphpad statistics software.• Laboratory material stock and ordering of lab supplies.• Biorepository Technician - Responsible for the collection of human tissue in various forms such as fresh tissue, fresh tissue in buffer, flash frozen, and FFPE material. Coordination between the study supervisors, pathologists, and courier services. Handling of sensitive health information and ensuring that the lab adhered to HIPPA and CAP regulations as well as internal record keeping -
Para-ProfessionalSoutheastern Massachusetts Educational Collaborative Sep 2013 - Aug 2015The Southcoast Of MassachussettsAs a 1-on-1 aid to an individual with autism at the beginning of 8th grade following to the high school level. I am responsible for school work, as well as long term behavior modification that needs to be made. Along with this individual, I have taken on the responsibility of teaching 8th grade science and freshman biology, also teaching math to a full classroom. • Created a science curriculum for the 8th grade and freshman science classes • Responsible for the well being of behaviorally challenged and intellectually delayed students • Teaching of math, biology, physics, and physical sciences at multiple grade levels
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Rare Disease Research Group InternPfizer Jul 2012 - Jul 2013Cambridge, MassachusettsI was a bench scientist, who was responsible for various complex assays and western blotting of samples, and analysis of the data. My responsibilities were broadened, to start a histology department within the lab. Sample preparation, staining, imaging and analysis. I also took part in the animal handling, including dosing, activity testing, and tissue harvesting. • Western Blotting, Complex Assays and ELISA assays • Cryosectioning, Immunohistochemical staining, immunoflourescent staining, Evans Blue Dye • In vivo animal work, including dosing and necropsy • Microscopy, Con-focal microscopy• Data analysis and Presentation -
Summer InternThe Schepens Eye Research Institute May 2011 - Jun 2011Boston, MassachusettsI joined a team working on immunology, specifically in the eye. Working with a mouse model, I began cryosectioning, staining and imaging tissue. I performed cell counting and number analysis on the images. • Microscopy • Tissue staining and immunohistochemistry • Data collection and analysis • “Conjunctival Inflammation in Thrombospondin-1 Deficient Mouse Model of Sjögren's Syndrome” in PLOS One Journal -
Histology InternArsenal Medical Aug 2009 - Jan 2010Watertown, MassachusettsMy first internship, I given the introduction to histology, learning hemotoxalin and eosin staining as well as Verhoeff van Gieson staining. I was responsible for the gross anatomy imaging and preparation of samples prior to staining. I was also responsible for weekly presentations of gross anatomy and histomophology data to a board of the senior scientists. • Tissue sampling technique • Sectioning techniques, mainly paraffin sectioning and cryosectioning • Traditional histo‐staining and Immunohistochemistry • Increased knowledge of experimental design and professional laboratory etiquette • Creation and delivery of professional presentations
James Kearns Skills
James Kearns Education Details
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Computation Biology And Bioinformatics -
Biotechnology -
Biology, General
Frequently Asked Questions about James Kearns
What company does James Kearns work for?
James Kearns works for Sanofi
What is James Kearns's role at the current company?
James Kearns's current role is Early-Stage Immunology Automation Lead – Vaccines.
What is James Kearns's email address?
James Kearns's email address is jk****@****ter.org
What is James Kearns's direct phone number?
James Kearns's direct phone number is +161763*****
What schools did James Kearns attend?
James Kearns attended University Of Delaware, Northeastern University, Northeastern University.
What are some of James Kearns's interests?
James Kearns has interest in Plant Biology, Children, Politics, Snowboarding, Biotechnology, Environment, Education, Research, Reading, Surfing.
What skills is James Kearns known for?
James Kearns has skills like Microsoft Office, Biotechnology, Histology, Microsoft Excel, Research, Immunohistochemistry, Powerpoint, Microsoft Word, Microscopy, Fluorescence Microscopy, Public Speaking, Teaching.
Who are James Kearns's colleagues?
James Kearns's colleagues are Ana Lilia Perez Ortiz, Celine Gennetay, Mouna Aissa, Ciro Masili, Voicu Elena, Linda Gadsden, Frederik Heinecke.
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