Jeffrey Patrick Email & Phone Number

Fishers, IN, US

Develop and implement mass spectrometric methods for the detection and potential quantitation of protein and peptide biomarkers associated with disease states. Systems include clinical samples and biological samples from model systems including in vitro (cell culture), CSF, muscle, bone and serum. Technologies include affinity and immunoaffinity capture.

Implemented targeted proteomic analysis in Biomarker analysis at Eli Lilly for trace level quantitative methods.. Interface with biologists and clinicians to develop and implement mass spectrometric methods for the detection and potential quantitation of protein and peptide biomarkers associated with disease states. Systems include clinical samples and.

Co-led a group in responsible for the characterization of peptides, proteins, antibodies and glycoproteins of biotechnology origin. Utilized mass sp[ectrometry, N-terminal sequence analysis, capillary electrophoresis and biochemical methods to achieve these results. Samples were from process, finished dosage forms and reference standards as we wll as.

Authored or reviewed technical reports for regulatroy submission for insulin and glucagon process upsets including identification or uknowns and characterization of the nature of the process upsetCreated, validated and provided comparability studies for new in-process, API and dosage form analytical methods for insulins, glucagon and other biomolecule.

Rebuilt group and co-led team of 12 to support characterization of antibodies, proteins and peptides from cell culture and process development through formulation development. Directed, developed and implemented experiments and method for the chemical characterization of peptides, proteins, antibodies and glycoproteins to support fermentation and cell.

Develop and implement experiments and methods for the chemical characterization of peptides, proteins, antibodies and glycoproteins to support fermentation and cell culture development, purification process development, API stability and drug product development and stability. Technologies include N-terminal sequencing, HPLC, capillary and gel.

Prepared and delivered lectures on protein analysis and chacarerization (mass spectrometry and capillary electrophoresis) to new program in Biotechnology including notes, labs and examsConsulted on program structure and format

Lead team responsible for chemical characterization of drug substance and finished drug (inhalation) product in support of CMC section of regulatory subsissions.Development and validation of analytical methods for drug product and drug substanceIdentify and characterize trace level degradants and impurities in metered dose inhalers and drug substance.

Leader of the team responsible for chemical characterization of drug substance and finished drug (inhalation) product in support of CMC section of regulatory subsissions. Implemented novel approaches for the identification and detection of impurities and degradants in metered dose inhalers leading to key deliverables for regulatory submissions. Provided.

Lead an anlytical group in support of formulations and process development. Develop/validate analytical methods for drug product and drug substance; monitor and interpret stability dataSupervise and train technicians and analysts in instrument operation and laboratory techniquesDirect and supervise technical group of up to 13 direct reportsRecommend.

Develop and validate analytical methods for the analysis and characterization of recombinant proteins and related biomolecules in fermentation, process and reference samples using protein chemistry, enzymology, HPLC, electrophoresis and GC*MS Identify trace level impurities in isolated drug product; supervise and train technicians in instrument operation.

Analysis of developmental dosage and process samples; develop and validate methods for the quantitative analysis of drugs, excipients and related compounds in dosage forms using HPLC and GC, as well as spectroscopic methods and wet chemistry.

Publications - more than 20 scientific and technical publicationsCommunity Activities

Phd, Analytical Chemistry

Ms, Biochemistry

Bs, Biochemistry

Doctorate, Analytical Chemistry

Advisor: Prof. R.G. Cooks Thesis: Thermochemical properties of biomolecules by MS; on-line analytical MS; Analysis using biomimetic surfaces

Master'S Of Science Degree; S.C, Analytical/Biochemistry

Advisor: Prof. J.W. Baynes Thesis: Identification and Quantitation of protein adducts in diabetic and senesent tissues

Bachelor'S Of Science Degree, Biochemistry