James Hall Email & Phone Number

San Diego, California, United States

Amplification and purification of DNA plasmids, along with purification of GMP antibody and protein products.

San Diego, California, United States

My work within the laboratory of Tatum Simonson focuses on the physiological, molecular, and genetic aspects of hypoxic response in high altitude adapted populations. The goal of this work is to better understand how the interplay between environment, genomes, and physiology shape human adaption and disease.

San Diego, California, United States

ANBI 134 - Human Evolutionary Genetics

Oak Ridge, Tn

Utilizing small angle X-ray and neutron scattering (SAXS/SANS) along with segmental labeling to elucidate intramolecular contacts and conformational changes for various conformational states of PKA and A kinase anchoring proteins (AKAPs).

La Jolla, Ca

The goal of my work in the laboratory of Dr. Susan Taylor is to elucidate how the dynamic structure of cAMP-dependent protein kinase (PKA) and its interactions with associated proteins contribute to regulation and function. Given the dynamic nature of the holoenzyme complex during resting state and catalytic turnover, elucidation of conformational changes between enzyme states in the holoenzyme and validation that the holoenzyme in solution reflects the current crystallographic data are current challenges in the field. Utilizing small angle X-ray and neutron scattering (SAXS/SANS) along with segmental labeling, intramolecular contacts and conformational changes for various states of the enzyme would be mapped. In addition, SAXS and electron microscopy can be used to characterize much larger complexes than are typically possible with X-ray crystallography. The characterization of the interactions between the PKA and A kinase anchoring proteins (AKAPs) within large macromolecular signaling complexes and the effects that modification (ie. phosphorylation or mutation) cause on these structures are highly relevant to understanding how PKA signaling is regulated and how disease states occur.

Lake Elsinore

Following SOPs and GMP to assure daily product quota is up to quality standards, packaged and prepared for shipment.

Research assistant in the Hille laboratory.• Structural and kinetic enzyme studies • Protein and small molecule purification and characterization.• Spectroscopy • Crystallography• Radiation Safety Contact for the Hille Lab.

Teaching assistant for BCH 102 ( Intro. Biochem. Lab) 3 quarters at UC Riverside• Support and assist students in running experiments and learning biochemistry laboratory techniques including spectrophotometry, pH and buffer preparation, methods of protein determination, principles and uses of chromatography, enzyme kinetic assays, theory and measurement of radioisotopes (liquid scintillation counting), SDS gel electrophoresis, and theory of centrifugation.Teaching assistant for BCH 101 ( Intro. Laboratory Fundamentals) 4 quarters at UC Riverside• Support and assist students in the use of molecular graphics programs for investigation of macromolecular structure-function relationships.• Support and assist students in learning biochemistry wet laboratory techniques for biological samples including micropipetting, volumetric relationships, dilutions, pH measurement, buffer preparation, spectrophotometry, gel permeation chromatography, and ion-exchange chromatography.Teaching assistant for BCH 100 (Elementary Biochemistry) 2 quarters at UC Riverside• Providing instructional and clerical support for the professors.• Running multiple discussion sections to support and assist students in learning the course material which includes an introduction to chemistry and molecular biology of living organisms based on a study of the structure, function, and metabolism of small molecules and macromolecules of biological significance while examining selected animals, plants, and microorganisms to develop a general understanding of structure-function relationships, enzyme action, regulation, bioenergetics, intermediary metabolism, and molecular biology.

Research assistant in the Spindler Drosophila lab at UC Riverside. • Maintaining cultures of Drosophila and separating populations of Drosophila based on sex and phenotype• Subjecting flies and mutant variants to a library of chemical targets, monitoring effects on lifespan.

Doctor Of Philosophy (Phd), Biochemistry And Molecular Biology

Master Of Science (Ms), Biochemistry And Molecular Biology

B.S., Biochemistry And Molecular Biology