John Hurley Email & Phone Number

Sterling, VA, US

Gaithersburg, Maryland, United States

• Support the NIST Engineering Biology program by executing experiments and overseeing general lab operations.
• Maintain laboratory equipment and general lab supplies/consumables, with minimal oversight.
• Works with engineering biology team to prepare engineered microbial strains and DNA plasmids.
• Includes growing and characterizing microorganisms and evaluating cellular properties.
• Prepares microbial culture media; sterilizes labware; performs DNA cloning and transformations;
• extracts nucleic acids for molecular detection methods including nest generation sequencing.

• Coordinate and execute travel plans to races and practices to ensure great athlete experience.
• Customize specific workout plans for middle distance, long distance, and sprinters.
• Collaborate and problem solve with other coaches to provide excellent coaching advice.

• Identified and articulated ethical considerations inherent to health analytics and data science.
• Created and used dataframes, functions, descriptive statistics, and sampling distributions in R to find percentages of certain genes in specific populations.
• Communicated the findings of how a sport enhancing oxygen saturation gene affects people by analyzing a 4,000 person sample with R. Presented data to multiple audiences including non-expert and researchers.

• Worked in BSL-2 laboratory with extesnive laboratory training.
• Research focus area: polyethylene glycol (PEG) plastic degrading ability of bacteria Pseudomonas putida, Bacillus subtilis, Klebsiella pneumoniae and fungus Aureobasidium pullulans.
• Designed and optimized procedures including sample preparation, data acquisition, and data analysis for raman spectroscopy and infrared Spectroscopy (IR) to assess PEG degradation.
• Monitored growth of bacteria and fungi on PEG utilizing a Cell ULTROSPEC to obtain optical density readings.
• Analyzed and interpreted spectroscopic data with LoggerPro software and Excel to generate meaningful conclusions that informed future experiments. Presented data to team.
• Awarded A.J Palumbo Grant for Research that recognizes high potential undergraduate student projects.

Latrobe, PA

• Mentored junior resident assistants, preparing them for their leadership journey
• Directed resident hall programs including team challenges, local excursions, and resident social and academic gatherings
• Developed a positive environment for hall members by engaging with them frequently to provide optimal physical and mental health conditions

• Expertise in RNA synthesis/extraction, DNA synthesis/purification, real-time PCR analysis of gene expression, protein assays plasmid design, restriction endonuclease mapping, diagnostic restriction enzyme analysis.
• Generated constructs with gene insertions using cloning vectors with DNA ligase enzyme. Constructs transformed into E.coli and selected with ampicillin resistant marker.
• Optimized pBluescript II KS- vector with restriction endonuclease sites and gene of interest for delivery into E.coli using SnapGene software.

20391 Lowes Island Blvd, Sterling, VA 20165

• Greeted new customers, discussed specials, and took drink orders.
• Bussed tables during busy periods to assist staff and quickly turn over tables.
• Displayed enthusiasm and promoted excellent service to customers, successfully increasing referrals, and walk-in business.
• Mowed and performed routine maintenance of putting greens, bunkers and fairways
• Took care of facility equipment, grounds, roads and fences.

Jamestown, Colorado, United States

• Facilitated rotating groups of middle-school and high-school students through weeklong program of adventure challenges, team building, and personal growth activities
• Mentored through servant leadership, collaborated with other counselors to plan and successfully complete outdoor adventure challenges in the Rocky Mountains
• Demonstrated leadership, communication skills, small group facilitation, catechesis, risk management, community building, and wilderness first aid

• Performed lipofection of ID2 gene into mouse myoblast cells, protein gel extraction, and protein purification.
• Cultured mouse myoblast cell line for three weeks and characterized percent confluency, dividing cells, floating vs. attached cells on daily basis to monitor the cell line’s health.
• Confirmed ID2 gene transfection with Blue-white screening to identify recombinant bacteria that were transfection positive.

19455 Deerfield Ave, Lansdowne, VA 20176

• Shadowed Doctors for initial consultations and diagnosis.
• Consolidated data from multiple sources representing surgical procedures performed by period, injury, and demographic.
• Documented and summarized data findings via short information blogs.
• Attended the NSMI Annual Sports Medicine Conference at Shenandoah University (2021 July).

• Monitored Swiss Webster mice to measure the capability of caffeine in combating sleep deprivation.
• Recorded pre-defined measurables and unique behavior to be later presented at research conference.

22015 Dulles Retail Plz, Sterling, VA 20166

• Serve 50+ guests daily at the front register and drive-thru, displaying hospitality to further Chick-fil-A’s values
• Offer food service support and report pressing issues to managers while promoting brand, culture, and standard of excellence
• Earned recognition for being on team taking over 200 car orders in 1 hour