Joseph Ong Email & Phone Number

San Diego, California, United States

Characterized AAVs and LVVs using a variety of techniques:-Capsid characterization: HPLC, LC-MS (peptide mapping with Waters QDa and Thermo Eclipse Tribrid), imaging capillary isoelectric focusing (cIEF); with a particular focus on capsid PTMS - deamidation, oxidation, glycosylation, and stability-indicating parameters-Genome integrity: denaturing alkaline gel DNA electrophoresis, charge electrophoresis (CE)-Empty/full ratio: analytical ultra centrifugation (AUC), charge detection mass spectrometry (CDMS, -Thermo Q Exactive UHMR), mass photometry

Workshop leader, 2018-present, HHMI Pathways (Supervisor: Tracy Johnson and London Williams) [4-6 hrs/week for 8 weeks]-Designed workshops for under-represented minority students in first-year chemistry (CHEM14A-D) to build an academic community and facilitate learning Writing Tutor, Summers 2019-present, Undergraduate Research Center - Sciences (Supervisor: Tama Hasson and Janet Goins) [8 hrs/week for 10 weeks]-Revised under-represented minority student writing assignments (scientific writing and graduate school preparation) Teaching Assistant, Winter 2017, CHEM153L (Supervisor: Anne Hong-Hermesdorf) [20 hours/week for 10 weeks]-Taught class of 20 students in fundamentals of biochemistry lab (E. coli protein purification, SDS-PAGE and western blotting, and enzymatic assays)

Project: The role of cytoskeletal elements during Drosophila oogenesis (Mentor: Margot Quinlan)-Generated flies of interest using traditional Drosophila genetics and animal husbandry techniques (Drosophila ovary dissections)-Characterized and assessed protein interactions (confocal microscopy, quantification of both fixed immunofluorescence and live microscopy, protein binding experiments)

Greater Los Angeles Area

Projects: Post-translational modifications in the regulation of cell division and cell cycle progression (Mentor: Jorge Torres)-Characterized relationships between Cullin-RING ubiquitin ligases, their substrates, and their substrate adaptors (tissue cell culture, RNA-interference with siRNA and shRNA, CRISPR/Cas9 knockout, qPCR, western blotting, molecular cloning to generate truncations and point mutants, binding assays, in vitro transcription and translation, transient transfections, co-immunoprecipitation, mass spectrometry, generation of doxycyline-inducible stable cell lines, immunofluorescence and time-lapse/live microscopy, flow cytometry, flow-assisted cell sorting [FACS], protein ubiquitylation and degradation experiments)-Identified novel substrates of ubiquitin ligases and kinases (autoradiography with 32P-ATP, protein purification, co-immunoprecipitation, mass spectrometry)-Constructed monoclonal SKOV3 ovarian cancer cell line expressing doxycyline-inducible Cas9 for CRISPR/Cas9 genetic screens-Presented work at many conferences (see CV for details)-Funded by: UCLA Division of Physical Sciences Pauley Fellowship, UCLA NIH T32 Cell and Molecular Biology Training Grant, NSF Graduate Research Fellowship, UCLA Whitcome MBI Pre-doctoral Fellowship

San Diego, California, United States

Supervisor: Yu Zhou, PhD-Characterized adeno-associated virus drug substances using analytical techniques (SDS-PAGE; in-gel digestion, LC-MS/MS, peptide mapping; N-terminal sequencing (Edman degradation); HPLC and intact and top-down mass spectrometry [Thermo Orbitrap Eclipse]; HPLC and amino acid analysis)-Presented work three times (two group meetings and one site-wide meeting)-Authored two technical reports

Notre Dame, In

Project: Using yeast as a biosensor for mutagenicity (Mentor: Holly Goodson)-Grew yeast in continuous culture systems as biosensor for mutagenicity (cultured, plated, and froze yeast; performed genomic DNA extraction, next generation sequencing, and bioinformatics analysis)-Presented research four times as a poster and three times as a platform presentation (2013-2016)-Awarded best science thesis (Glynn Family Honors Program, April 2016)-Mentored 6 undergraduate engineering students (Summer 2016)-Published as first-author paper in PLOS One (2021)

Singapore

Project: Immortalization of human skeletal muscle cells (Mentor: Shyh-Chang Ng)-Immortalized human skeletal muscle stem cells (tissue cell culture, molecular cloning, generating lentiviral vectors)-Assessed cell lines for stem cell potential (qPCR, western blotting, cell growth assays, microscopy)-Presented research once as a poster (2015)-Funded by: Singapore International Pre-Graduate Award [A*STAR, Singapore]; Career Center, Glynn Family Honors Program, MSPS [University of Notre Dame])

Dallas, Texas

Project: Isolation of the endogenous ligand of the PAS-B domain of HIF-2a (Mentor: John MacMillan)-Isolated natural products from mammalian tissues (liquid-phase extraction and solid-phase extraction), analyzed resulting natural products via various analytical techniques (NMR, LC-MS)-Performed steps in organic synthesis of enzyme inhibitor-Presented research twice as a poster (2014)-Funded by: UTSW Summer Undergraduate Research Fellowship program

Doctor Of Philosophy (Ph.D.), Biochemistry, Molecular And Structural Biology

Bachelor’S Degree, Chemistry (Honors Track), Cum Laude

Secondary Major: Chinese; Minor: Glynn Family Honors Program

Ay1415 Semester 2 Non-Graduating Exchange, Chemistry