Lisa Hughes Email & Phone Number

Philadelphia, PA, US

West Chester, PA

• Formulated proteins and small molecules for solubility and stability in solution and in combination products.
• Developed discriminatory in vitro release method to detect differences in formulations pertaining to API, carrier, and API-carrier combination.
• Involved in preclinical studies including API and combination product formulations, preparation, and on site execution. Performed analysis of ex vivo samples.
• Led collaborations regarding materials development with external… Show more
• Led collaborations regarding materials development with external partners including research institutes, pharmaceutical partners, and contract organizations
• Lead team of internal and external scientists on the development of a combination product for local bone healing involving a small molecule API and synthetic carrier.

West Chester, PA

• Isolated, cultured, and differentiated Mesenchymal Stem Cell (MSC) to osteoblasts. Performed bioassays to measure differentiation process via alkaline phosphatase expression or mineralization.
• Performed protein radiolabeling with 125I. Developed methods for measuring protein binding and release kinetics using radiolabeled proteins.
• Authorized user for 125I and 3H. Responsible for hands-on training of new users and radiation safety training for auxiliary staff. Completed DOT… Show more
• Authorized user for 125I and 3H. Responsible for hands-on training of new users and radiation safety training for auxiliary staff. Completed DOT training for shipping of radioactive materials and instruments.
• Performed bioassays including immunofluorochemistry, cytotoxicity, ELISA, and protein concentration.
• Serve as SME on Fluorescence based techniques including Fluorescence Activated Cell Sorting (FACS) and Epifluorescence and confocal microscopy using immunohistochemical and fluorescent staining.

Gaithersburg, MD

• Correlated cell response to mechanical deformation.
• Modified hydrophobic surfaces for cell attachment and proliferation using proteins. Surfaces were physically characterized for protein presence and coverage and hydrophobicity and biologically characterized for cell.
• various characterization techniques: FT-IR, confocal, fluorescence, and scanning electon microscopies, and contact angle.
• Performed bioassasys including real time polymerase chain… Show more
• Performed bioassasys including real time polymerase chain reaction (RT-PCR), BrdU, and immunochemical staining to measure and understand cell response.
• Focused on advanced imaging techniques and understanding of cell behavior in 2D and 3D environments for tissue engineering applications. Show less

Newark, DE

• Characterized amphiphilic diblock polypeptides that controllably self-assemble into innately porous hydrogels, twisted fibrils, single crystals, and vesicles for biomedical applications.
• Techniques: transmission electron (TEM), cryo-TEM, optical, atomic force (AFM), and laser scanning confocal microscopies; small angle x-ray and neutron scattering; rheology; DNA electrophoresis; circular dichroism (CD).
• Tested hydrogel biocompatibility using bacterial and mammalian cell systems.… Show more
• Tested hydrogel biocompatibility using bacterial and mammalian cell systems.
• Designed and produced analogous polypeptides using recombinant DNA methods.
• Long term focus: understand diblock copolypeptide assembly and properties to tailor materials for specific applications.

Phd, Materials Science And Engineering

Bs, Chemistry

Research in biochemistry and biomimetic chemistry, including inorganic mineral synthesis within biological systems of constricted size and.